Product Name
HOXD13, siRNA
Full Product Name
HOXD13 siRNA (Human)
Product Synonym Names
HOX4I; Homeobox protein Hox-D13; Homeobox protein Hox-4I
Product Gene Name
HOXD13 sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P35453
Specificity
HOXD13 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human HOXD13 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of HOXD13 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
HOXD13 sirna
siRNA to inhibit HOXD13 expression using RNA interference
Applications Tested/Suitable for HOXD13 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for HOXD13. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000514.2
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NCBI GenBank Nucleotide #
NM_000523.3
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UniProt Primary Accession #
P35453
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UniProt Related Accession #
P35453[Other Products]
Molecular Weight
36,101 Da
NCBI Official Full Name
homeobox protein Hox-D13
NCBI Official Synonym Full Names
homeobox D13
NCBI Official Symbol
HOXD13 [Similar Products]
NCBI Official Synonym Symbols
BDE; SPD; BDSD; HOX4I
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NCBI Protein Information
homeobox protein Hox-D13
UniProt Protein Name
Homeobox protein Hox-D13
UniProt Synonym Protein Names
Homeobox protein Hox-4I
Protein Family
Homeobox protein
UniProt Gene Name
HOXD13 [Similar Products]
UniProt Synonym Gene Names
HOX4I [Similar Products]
UniProt Entry Name
HXD13_HUMAN
NCBI Summary for HOXD13
This gene belongs to the homeobox family of genes. The homeobox genes encode a highly conserved family of transcription factors that play an important role in morphogenesis in all multicellular organisms. Mammals possess four similar homeobox gene clusters, HOXA, HOXB, HOXC and HOXD, located on different chromosomes, consisting of 9 to 11 genes arranged in tandem. This gene is one of several homeobox HOXD genes located in a cluster on chromosome 2. Deletions that remove the entire HOXD gene cluster or the 5' end of this cluster have been associated with severe limb and genital abnormalities. Mutations in this particular gene cause synpolydactyly. [provided by RefSeq, Jul 2008]
UniProt Comments for HOXD13
HOXD13: Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. Defects in HOXD13 are the cause of synpolydactyly 1 (SPD1); also known as syndactyly type 2 (SDTY2). SPD1 is a limb malformation that shows a characteristic manifestation in both hands and feet. This condition is inherited as an autosomal dominant trait with reduced penetrance. Defects in HOXD13 are the cause of brachydactyly type D (BDD). BDD is characterized by short and broad terminal phalanges of the thumbs and big toes. Inheritance is autosomal dominant. Defects in HOXD13 are the cause of syndactyly type 5 (SDTY5); also known as syndactyly with metacarpal and metatarsal fusion. The metacarpals and metatarsals most commonly fused are the 4th and 5th or the 3rd and 4th. Soft tissue syndactyly usually affects the 3rd and 4th fingers and the 2nd and 3rd toes. Inheritance is autosomal dominant. Defects in HOXD13 are the cause of brachydactyly- syndactyly syndrome (BDSD). Most of affected individuals exhibit generalized shortening of the hands and feet, broad and short distal phalanges of the thumbs, and cutaneous syndactyly of toes 2 and 3. The limb phenotypes observed in this syndrome overlap those of brachydactyly types A4, D, E and syndactyly type 1. Defects in HOXD13 are the cause of brachydactyly type E (BDE1). BDE is characterized by shortening of the fingers mainly in the metacarpals and metatarsals. Inheritance is autosomal dominant. Defects in HOXD13 are a cause of VACTERL association (VACTERL); which includes also VATER association. VACTERL is an acronym for vertebral anomalies, anal atresia, congenital cardiac disease, tracheoesophageal fistula, renal anomalies, radial dysplasia, and other limb defects. Belongs to the Abd-B homeobox family.
Protein type: DNA-binding
Chromosomal Location of Human Ortholog: 2q31.1
Cellular Component: nucleus
Molecular Function: DNA binding; sequence-specific DNA binding; chromatin binding; transcription factor activity
Biological Process: anterior/posterior pattern formation; transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; multicellular organismal development; male genitalia development; positive regulation of transcription from RNA polymerase II promoter; embryonic digit morphogenesis; skeletal development; regulation of cell proliferation; embryonic hindgut morphogenesis
Disease: Synpolydactyly 1; Brachydactyly-syndactyly Syndrome; Brachydactyly, Type E1; Syndactyly, Type V; Brachydactyly, Type D; Vater Association
Research Articles on HOXD13
1. Linkage analysis of the syndactyly type 1 subtype c (SD1-c) phenotype based on two Chinese families with 3/4 fingers syndactyly shows that two missense mutations in codon 306 of HOXD13 underlie SD1-c.
Precautions
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