Product Name
DMGDH, siRNA
Full Product Name
DMGDH siRNA (Human)
Product Synonym Names
Dimethylglycine dehydrogenase mitochondrial; ME2GLYDH
Product Gene Name
DMGDH sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q9UI17
Specificity
DMGDH siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human DMGDH gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of DMGDH sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
DMGDH sirna
siRNA to inhibit DMGDH expression using RNA interference
Applications Tested/Suitable for DMGDH sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for DMGDH. It may not necessarily be applicable to this product.
NCBI Accession #
NP_037523.2
[Other Products]
NCBI GenBank Nucleotide #
NM_013391.3
[Other Products]
UniProt Primary Accession #
Q9UI17
[Other Products]
UniProt Secondary Accession #
B2RBN0; B4E1J9[Other Products]
UniProt Related Accession #
Q9UI17[Other Products]
Molecular Weight
45,079 Da
NCBI Official Full Name
dimethylglycine dehydrogenase, mitochondrial
NCBI Official Synonym Full Names
dimethylglycine dehydrogenase
NCBI Official Symbol
DMGDH [Similar Products]
NCBI Official Synonym Symbols
DMGDHD; ME2GLYDH
[Similar Products]
NCBI Protein Information
dimethylglycine dehydrogenase, mitochondrial
UniProt Protein Name
Dimethylglycine dehydrogenase, mitochondrial
UniProt Synonym Protein Names
ME2GLYDH
Protein Family
Dimethylglycine dehydrogenase
UniProt Gene Name
DMGDH [Similar Products]
UniProt Entry Name
M2GD_HUMAN
NCBI Summary for DMGDH
This gene encodes an enzyme involved in the catabolism of choline, catalyzing the oxidative demethylation of dimethylglycine to form sarcosine. The enzyme is found as a monomer in the mitochondrial matrix, and uses flavin adenine dinucleotide and folate as cofactors. Mutation in this gene causes dimethylglycine dehydrogenase deficiency, characterized by a fishlike body odor, chronic muscle fatigue, and elevated levels of the muscle form of creatine kinase in serum. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]
UniProt Comments for DMGDH
DMGDH: Defects in DMGDH are the cause of DMGDH deficiency (DMGDHD). DMGDHD is a disorder characterized by fish odor, muscle fatigue with increased serum creatine kinase. Biochemically it is characterized by an increase of N,N- dimethylglycine (DMG) in serum and urine. Belongs to the GcvT family.
Protein type: Mitochondrial; Amino Acid Metabolism - glycine, serine and threonine; EC 1.5.8.4; Oxidoreductase
Chromosomal Location of Human Ortholog: 5q14.1
Cellular Component: mitochondrial matrix
Molecular Function: electron carrier activity; dimethylglycine dehydrogenase activity
Biological Process: glycine metabolic process; choline metabolic process; betaine catabolic process
Disease: Dimethylglycine Dehydrogenase Deficiency
Research Articles on DMGDH
1. Observational study of gene-disease association and gene-gene interaction. (HuGE Navigator)
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
