Full Product Name
DMGDH Antibody
Product Synonym Names
Dimethylglycine dehydrogenase, mitochondrial; ME2GLYDH; DMGDH
Product Gene Name
anti-DMGDH antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q9UI17
Species Reactivity
Human, Mouse
Purity/Purification
>95%, Protein G purified
Immunogen
Recombinant Human Dimethylglycine dehydrogenase, mitochondrial protein (429-524AA)
Conjugation
Non-conjugated
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Preparation and Storage
Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Other Notes
Small volumes of anti-DMGDH antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-DMGDH antibody
Catalyzes the demethylation of N, N-dimethylglycine to sarcosine. Also has activity with sarcosine in vitro.
Product Categories/Family for anti-DMGDH antibody
Neuroscience; Metabolism; Signal transduction
Applications Tested/Suitable for anti-DMGDH antibody
ELISA (EIA), Western Blot (WB), Immunofluorescence (IF)
Application Notes for anti-DMGDH antibody
WB: 1:500-1:5000
IF: 1:50-1:200
Western Blot (WB) of anti-DMGDH antibody
Western Blot
Positive WB detected in: Mouse liver tissue, Mouse kidney tissue
All lanes: DMGDH antibody at 6.7ug/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 97, 46 KDa
Observed band size: 97 KDa
Immunofluorescence (IF) of anti-DMGDH antibody
Immunofluorescence staining of HepG2 cells with MBS7107643 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
NCBI/Uniprot data below describe general gene information for DMGDH. It may not necessarily be applicable to this product.
NCBI Accession #
NP_037523.2
[Other Products]
NCBI GenBank Nucleotide #
NM_013391.3
[Other Products]
UniProt Primary Accession #
Q9UI17
[Other Products]
UniProt Secondary Accession #
B2RBN0; B4E1J9[Other Products]
UniProt Related Accession #
Q9UI17[Other Products]
Molecular Weight
45,079 Da
NCBI Official Full Name
dimethylglycine dehydrogenase, mitochondrial
NCBI Official Synonym Full Names
dimethylglycine dehydrogenase
NCBI Official Symbol
DMGDH [Similar Products]
NCBI Official Synonym Symbols
DMGDHD; ME2GLYDH
[Similar Products]
NCBI Protein Information
dimethylglycine dehydrogenase, mitochondrial
UniProt Protein Name
Dimethylglycine dehydrogenase, mitochondrial
UniProt Synonym Protein Names
ME2GLYDH
Protein Family
Dimethylglycine dehydrogenase
UniProt Gene Name
DMGDH [Similar Products]
NCBI Summary for DMGDH
This gene encodes an enzyme involved in the catabolism of choline, catalyzing the oxidative demethylation of dimethylglycine to form sarcosine. The enzyme is found as a monomer in the mitochondrial matrix, and uses flavin adenine dinucleotide and folate as cofactors. Mutation in this gene causes dimethylglycine dehydrogenase deficiency, characterized by a fishlike body odor, chronic muscle fatigue, and elevated levels of the muscle form of creatine kinase in serum. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]
UniProt Comments for DMGDH
Catalyzes the demethylation of N,N-dimethylglycine to sarcosine. Also has activity with sarcosine in vitro.
Research Articles on DMGDH
1. In nonalcoholic fatty liver disease patient, the missense variant p.Ser646Pro (rs1805074) in DMGDH gene was significantly associated with disease severity and circulating levels of dimethylglycine.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
