Full Product Name
GLI-3 Antibody
Product Synonym Names
ACLS; GCPS; GLI-Kruppel family member GLI3 (Greig cephalopolysyndactyly syndrome); PAP-A; PAPA; PAPA1; PAPB; PHS; PPDIV
Product Gene Name
anti-GLI-3 antibody
[Similar Products]
Product Synonym Gene Name
GLI3[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P10071
Species Reactivity
Human, Mouse, Rat
Specificity
GLI-3 Antibody detects endogenous levels of total GLI-3 protein.
Purity/Purification
Purified from rabbit antiserum by affinity-chromatography using immunogen.
Form/Format
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1 mg/ml (lot specific)
Target Modification
Unmodified/Total
Immunogen
The antiserum was produced against synthesized peptide derived from human GLI-3.
Preparation and Storage
Stable at -20 degree C for at least 1 year.
Other Notes
Small volumes of anti-GLI-3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-GLI-3 antibody
Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)
Application Notes for anti-GLI-3 antibody
IHC: 1:50~1:100
IF: 1:100~1:500
ELISA: 1:10000
Immunofluorescence (IF) of anti-GLI-3 antibody
Immunofluorescence analysis of HepG2 cells, using GLI-3 Antibody. The picture on the right is blocked with the synthesized peptide.
Immunohistochemistry (IHC) of anti-GLI-3 antibody
Immunohistochemistry analysis of paraffin-embedded human brain tissue, using GLI-3 Antibody. The picture on the right is blocked with the synthesized peptide.
NCBI/Uniprot data below describe general gene information for GLI-3. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000159.3
[Other Products]
NCBI GenBank Nucleotide #
NM_000168.5
[Other Products]
UniProt Primary Accession #
P10071
[Other Products]
UniProt Secondary Accession #
O75219; Q17RW4; Q75MT0; Q75MU9; Q9UDT5; Q9UJ39; A4D1W1[Other Products]
UniProt Related Accession #
P10071[Other Products]
NCBI Official Full Name
transcriptional activator GLI3
NCBI Official Synonym Full Names
GLI family zinc finger 3
NCBI Official Symbol
GLI3 [Similar Products]
NCBI Official Synonym Symbols
PHS; ACLS; GCPS; PAPA; PAPB; PAP-A; PAPA1; PPDIV; GLI3FL; GLI3-190
[Similar Products]
NCBI Protein Information
transcriptional activator GLI3
UniProt Protein Name
Transcriptional activator GLI3
UniProt Synonym Protein Names
GLI3 form of 190 kDa; GLI3-190
UniProt Gene Name
GLI3 [Similar Products]
UniProt Synonym Gene Names
GLI3-190; GLI3FL; GLI3-83 [Similar Products]
NCBI Summary for GLI-3
This gene encodes a protein which belongs to the C2H2-type zinc finger proteins subclass of the Gli family. They are characterized as DNA-binding transcription factors and are mediators of Sonic hedgehog (Shh) signaling. The protein encoded by this gene localizes in the cytoplasm and activates patched Drosophila homolog (PTCH) gene expression. It is also thought to play a role during embryogenesis. Mutations in this gene have been associated with several diseases, including Greig cephalopolysyndactyly syndrome, Pallister-Hall syndrome, preaxial polydactyly type IV, and postaxial polydactyly types A1 and B. [provided by RefSeq, Jul 2008]
UniProt Comments for GLI-3
Has a dual function as a transcriptional activator and a repressor of the sonic hedgehog (Shh) pathway, and plays a role in limb development. The full-length GLI3 form (GLI3FL) after phosphorylation and nuclear translocation, acts as an activator (GLI3A) while GLI3R, its C-terminally truncated form, acts as a repressor. A proper balance between the GLI3 activator and the repressor GLI3R, rather than the repressor gradient itself or the activator/repressor ratio gradient, specifies limb digit number and identity. In concert with TRPS1, plays a role in regulating the size of the zone of distal chondrocytes, in restricting the zone of PTHLH expression in distal cells and in activating chondrocyte proliferation. Binds to the minimal GLI-consensus sequence 5'-GGGTGGTC-3'.
Research Articles on GLI-3
1. The c.480dupC of the GLI3 gene probably underlies the synpolydactyly in this family.
Precautions
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Disclaimer
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