PRPF8, Polyclonal Antibody

PRP8; RP13; HPRP8; PRPC8; SNRNP220

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

IgG

Rabbit

Affinity Purification

1mg/ml (lot specific)

Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Small volumes of anti-PRPF8 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Pre-mRNA splicing occurs in 2 sequential transesterification steps. The protein encoded by this gene is a component of both U2- and U12-dependent spliceosomes, and found to be essential for the catalytic step II in pre-mRNA splicing process. It contains several WD repeats, which function in protein-protein interactions. This protein has a sequence similarity to yeast Prp8 protein. This gene is a candidate gene for autosomal dominant retinitis pigmentosa.

Polyclonal

Western Blot (WB), Immunoprecipitation (IP)

WB: 1:500 - 1:1000
IP: 1:20 - 1:50

Western blot analysis of extracts of various cell lines, using PRPF8 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.

Immunohistochemistry of paraffin-embedded human esophagus using PRPF8 antibody at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat brain using PRPF8 antibody at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse stomach using PRPF8 antibody at dilution of 1:100 (40x lens).

2335

pre-mRNA processing factor 8

pre-mRNA-processing-splicing factor 8; p220; PRP8 homolog; splicing factor Prp8; apoptosis-regulated protein 1; apoptosis-regulated protein 2; 220 kDa U5 snRNP-specific protein; precursor mRNA processing protein; PRP8 pre-mRNA processing factor 8 homolog; U5 snRNP-specific protein (220 kD), ortholog of S. cerevisiae Prp8p

Pre-mRNA-processing-splicing factor 8

PRPC8  [Similar Products]

PRP8_HUMAN

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.