11beta-HSD1, Polyclonal Antibody

HSD11B1; HSD11; HSD11L; Corticosteroid 11-beta-dehydrogenase isozyme 1; 11-beta-hydroxysteroid dehydrogenase 1; 11-DH; 11-beta-HSD1

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

IgG

Rabbit

Antigen Affinity Purified

1 mg/ml (lot specific)

Store at -20 degree C. Avoid freeze / thaw cycles.

Manufactured in an ISO 9001:2015 Certified Laboratory.

Small volumes of anti-11beta-HSD1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Cancer

Western Blot (WB), ELISA (EIA)

WB: 1:500-1:2000, ELISA: 1:5000-1:20000

Western Blot analysis of K562 cells using 11?-HSD1 Polyclonal Antibody at dilution of 1:500.

Immunohistochemistry of paraffin-embedded Human liver tissue using 11?-HSD1 Polyclonal Antibody at dilution of 1:100.

Immunohistochemistry of paraffin-embedded Human brain tissue using 11?-HSD1 Polyclonal Antibody at dilution of 1:100.

Calculated MW: 32
Observed MW: 35

Corticosteroid 11-beta-dehydrogenase isozyme 1

Hsd11; 11-DH; 11-beta-HSD1  [Similar Products]

DHI1_RAT

HSD11B1: Catalyzes reversibly the conversion of cortisol to the inactive metabolite cortisone. Catalyzes reversibly the conversion of 7-ketocholesterol to 7-beta-hydroxycholesterol. In intact cells, the reaction runs only in one direction, from 7- ketocholesterol to 7-beta-hydroxycholesterol. Defects in HSD11B1 are a cause of cortisone reductase deficiency (CRD). In CRD, activation of cortisone to cortisol does not occur, resulting in adrenocorticotropin-mediated androgen excess and a phenotype resembling polycystic ovary syndrome (PCOS). Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Protein type: Membrane protein, integral; Oxidoreductase; Transferase; Endoplasmic reticulum; RNA-binding; DNA replication; Nuclear envelope; Lipid Metabolism - C21-steroid hormone; EC 1.1.1.146; Lipid Metabolism - androgen and estrogen

Cellular Component: apical part of cell; cytoplasm; endoplasmic reticulum membrane; integral to membrane; intracellular membrane-bound organelle; membrane; nuclear membrane; rough endoplasmic reticulum

Molecular Function: 11-beta-hydroxysteroid dehydrogenase activity; NADP binding; oxidoreductase activity; steroid binding

Biological Process: glucocorticoid biosynthetic process; glucocorticoid catabolic process; lung development; phthalate metabolic process; regulation of pentose-phosphate shunt; response to nutrient levels

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