Calcium Sensing Receptor, Polyclonal Antibody

Rabbit Anti-Human Calcium Sensing Receptor Polyclonal; Hypocalciuric hypercalcemia 1 severe neonatal hyperparathyroidism Antibody; HHC Antibody; Extracellular calcium sensing receptor Antibody; FIH Antibody; Parathyroid Cell calcium-sensing receptor Antibody; CASR Antibody; CASR_HUMAN Antibody; Ca sensing receptor Antibody; Extracellular calcium-sensing receptor Antibody; PCAR1 Antibody; FHH Antibody; Ca2+ sensing receptor Antibody; EIG8 Antibody; Hypocalciuric hypercalcemia 1 Antibody; PCaR1 Antibody; GPRC2A Antibody; Extracellular calcium sensing receptor [Precursor] Antibody; PCAR 1 Antibody; Parathyroid cell calcium-sensing receptor 1 Antibody; HHC1 Antibody; Parathyroid Cell calcium sensing receptor Antibody; CAR Antibody; MGC138441 Antibody; NSHPT Antibody; CaSR Antibody; Ca2+ sensing receptor 1 Antibody; Parathyroid Ca(2+) sensing receptor 1 Antibody

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit

Detects ~140 kDa.

Peptide Affinity Purified

1 mg/ml (lot specific)

Store at -20 degree C.

Small volumes of anti-CASR antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Antibodies; Neuroscience; Neurotransmission; Neurotransmitter Receptors; Glutamate Receptors; Cell Signaling; Cytoskeleton; Cancer; Cancer Metabolism

Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)

WB: 1:1000
ICC/IF: 1:100

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Calcium Sensing Receptor Polyclonal Antibody (SPC-713). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-Calcium Sensing Receptor Polyclonal Antibody (SPC-713) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell Membrane, Multi-Pass Membrane Protein. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Calcium Sensing Receptor Antibody (D) Composite.

Western blot analysis of Rat Kidney cell lysates showing detection of 130kDa Calcium Sensing Receptor protein using Rabbit Anti-Calcium Sensing Receptor Polyclonal Antibody (SPC-713). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Kidney cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Calcium Sensing Receptor Polyclonal Antibody (SPC-713) at 1:1000 for 16 hours at 4 degree C. Secondary Antibody: Goat-Anti-Rabbit IgG: HRP at 1:200 for 60 min at RT. Color Development: TMB. Predicted/Observed Size: 130kDa.

121,773 Da

calcium sensing receptor

extracellular calcium-sensing receptor

Extracellular calcium-sensing receptor

PCaR1  [Similar Products]

The protein encoded by this gene is a plasma membrane G protein-coupled receptor that senses small changes in circulating calcium concentration. The encoded protein couples this information to intracellular signaling pathways that modify parathyroid hormone secretion or renal cation handling, and thus this protein plays an essential role in maintaining mineral ion homeostasis. Mutations in this gene are a cause of familial hypocalciuric hypercalcemia, neonatal severe hyperparathyroidism, and autosomal dominant hypocalcemia. [provided by RefSeq, Aug 2017]

G-protein-coupled receptor that senses changes in the extracellular concentration of calcium ions and plays a key role in maintaining calcium homeostasis (PubMed:7759551, PubMed:8702647, PubMed:8636323, PubMed:8878438, PubMed:17555508, PubMed:19789209, PubMed:21566075, PubMed:22114145, PubMed:23966241, PubMed:25292184, PubMed:25104082, PubMed:26386835, PubMed:25766501, PubMed:22789683). Senses fluctuations in the circulating calcium concentration and modulates the production of parathyroid hormone (PTH) in parathyroid glands (). The activity of this receptor is mediated by a G-protein that activates a phosphatidylinositol-calcium second messenger system (PubMed:7759551). The G-protein-coupled receptor activity is activated by a co-agonist mechanism: aromatic amino acids, such as Trp or Phe, act concertedly with divalent cations, such as calcium or magnesium, to achieve full receptor activation (PubMed:27434672, PubMed:27386547).

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