3-mercaptopyruvate sulfurtransferase, ELISA Kit

MGC24539; MST; TST2; 3-mercaptopyruvate sulfurtransferase|OTTHUMP00000197788|OTTHUMP00000197791|human liver rhodanese

For Research Use Only. Not for use in diagnostic procedures.

This assay has high sensitivity and excellent specificity for detection of Rat MPST. No significant cross-reactivity or interference between Rat MPST and analogues was observed.

Store at 2-8 degree C.

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of MPST elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS284362 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the 3-mercaptopyruvate sulfurtransferase (MPST) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing MPST. The ELISA analytical biochemical technique of the MBS284362 kit is based on MPST antibody-MPST antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MPST antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MPST. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate MPST in samples. An antibody specific for MPST has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMPST present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MPST is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MPST bound in the initial step. The color development is stopped and the intensity of the color is measured.

32,940 Da

mercaptopyruvate sulfurtransferase

3-mercaptopyruvate sulfurtransferase

3-mercaptopyruvate sulfurtransferase

MST  [Similar Products]

catalyzes the formation of thiocyanide and pyruvate [RGD, Feb 2006]

MPST: Transfer of a sulfur ion to cyanide or to other thiol compounds. Also has weak rhodanese activity. May have a role in cyanide degradation or in thiosulfate biosynthesis.

Protein type: Amino Acid Metabolism - cysteine and methionine; EC 2.8.1.2; Mitochondrial; Transferase

Chromosomal Location of Human Ortholog: 7q34

Cellular Component: cell junction; cytoplasm; cytosol; mitochondrial inner membrane; mitochondrion; neuron projection; synapse

Molecular Function: 3-mercaptopyruvate sulfurtransferase activity; identical protein binding; thiosulfate sulfurtransferase activity

Biological Process: kidney development; liver development; spinal cord development; transsulfuration

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