TAR DNA-binding protein 43, Polyclonal Antibody

TDP-43; TARDBP; TDP43

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

IgG

Rabbit

Antigen Affinity Purified

Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Small volumes of anti-TARDBP antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

DNA and RNA-binding protein which regulates transcription and splicing. Involved in the regulation of CFTR splicing. It promotes CFTR exon 9 skipping by binding to the UG repeated motifs in the polymorphic region near the 3'-splice site of this exon. The resulting aberrant splicing is associated with pathological features typical of cystic fibrosis. May also be involved in microRNA biogenesis, apoptosis and cell division. Can repress HIV-1 transcription by binding to the HIV-1 long terminal repeat. Stabilizes the low molecular weight neurofilament (NFL) mRNA through a direct interaction with the 3' UTR.

ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)

Recommended dilution: WB:1:500-5000
IHC:1:20-1:200

All lanes: TAR DNA-binding protein 43 antibody at 8ug/ml
Lane 1:Hela whole cell lysate
Lane 2:mouse spleen tissue
Lane:K562 whole cell lysate
Secondary
Goat polyclonal to rabbit at 1/10000 dilution
Predicted band size: 45,32 kDa
Observed band size: 45 kDa

Immunohistochemistry of paraffin-embedded human breast cancer using MBS1497439 at dilution 1:100

Immunohistochemistry of paraffin-embedded human placenta using MBS1497439 at dilution 1:100

Immunoprecipitating TARDBP in Hela whole cell lysate
Lane 1: Rabbit control IgG (1ug)instead of MBS1497439 in Hela whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: MBS1497439 (8ug)+ Hela whole cell lysate (500ug)
Lane 3: Hela whole cell lysate (10ug)

31,808 Da

TAR DNA binding protein

TAR DNA-binding protein 43

TAR DNA-binding protein 43

TDP43; TDP-43  [Similar Products]

TADBP_HUMAN

HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. The protein encoded by this gene is a transcriptional repressor that binds to chromosomally integrated TAR DNA and represses HIV-1 transcription. In addition, this protein regulates alternate splicing of the CFTR gene. A similar pseudogene is present on chromosome 20. [provided by RefSeq, Jul 2008]

TARDBP: DNA and RNA-binding protein which regulates transcription and splicing. Involved in the regulation of CFTR splicing. It promotes CFTR exon 9 skipping by binding to the UG repeated motifs in the polymorphic region near the 3'-splice site of this exon. The resulting aberrant splicing is associated with pathological features typical of cystic fibrosis. May also be involved in microRNA biogenesis, apoptosis and cell division. Can repress HIV-1 transcription by binding to the HIV-1 long terminal repeat. Stabilizes the low molecular weight neurofilament (NFL) mRNA through a direct interaction with the 3' UTR. Homodimer. Binds specifically to pyrimidine-rich motifs of TAR DNA and to single stranded TG repeated sequences. Binds to RNA, specifically to UG repeated sequences with a minimun of six contiguous repeats. Interacts with ATNX2; the interaction is RNA-dependent. Ubiquitously expressed. In particular, expression is high in pancreas, placenta, lung, genital tract and spleen. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA-binding

Chromosomal Location of Human Ortholog: 1p36.22

Cellular Component: cytoplasm; nuclear speck; nucleoplasm; nucleus; perichromatin fibrils

Molecular Function: double-stranded DNA binding; identical protein binding; mRNA 3'-UTR binding; nucleotide binding; protein binding; RNA binding; transcription factor activity

Biological Process: mRNA processing; negative regulation of protein amino acid phosphorylation; nuclear fragmentation during apoptosis; positive regulation of insulin secretion; positive regulation of transcription from RNA polymerase II promoter; regulation of cell cycle; RNA splicing; transcription from RNA polymerase II promoter

Disease: Amyotrophic Lateral Sclerosis 10, With Or Without Frontotemporal Dementia

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