CD99-L2, Polyclonal Antibody

Anti -CD99-L2 (CD99 antigen-like 2) (Biotin)

For Research Use Only. Not for use in diagnostic procedures.

Chromosome: X; NC_000023.10 (2609228..2659350). Location: Xp22.32 and Yp11.3

Polyclonal

IgG

Goat

Recognizes mouse CD99-L2, extracellular domain. Shows approximately 10% cross-reactivity with rhCD99-L2 and less than 5% cross-reactivity with rmCD99.

Affinity Purified
Purified by immunoaffinity chromatography.

Supplied as lyophilized from a 0.2um sterile-filtered solution in PBS containing 50ug of BSA per 1ug of antibody. Reconstitute with sterile Tris-buffered saline pH 7.3 (20mM Tris base,

Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute by adding sterile 40-50% glycerol, aliquot and store at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Small volumes of anti-CD99 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

CD99 antigen-like 2 (CD99-L2) is a 45kD type I transmembrane glycoprotein in the CD99 family of molecules (1-3). Mouse CD99-L2 cDNA encodes a 214 amino acid (aa) precursor with a 23 aa predicted signal sequence, a 116 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 54 aa cytoplasmic region (4). The ECD contains no N-linked glycosylation sites, but O-linked glycosylation is likely (1, 2). A long isoform with a 23 aa insert after aa 43 within the ECD is expressed in human, and probably in mouse (2, 5). Both long and short isoforms may have minor variants missing portions of the N-terminus of the mature protein (6). The ECD of mouse CD99-L2 short isoform shares 85%, 72% and 66% aa identity with rat, human, and bovine CD99-L2, respectively (3, 5). The mouse CD99 and CD99-L2 ECDs share only 31% aa identity, but both contain three conserved acidic motifs and are thought to originate from the same ancestral gene (1, 2). The nearly ubiquitous expression of CD99-L2 is similar to that of CD99. Mouse CD99-L2 shows highest in situ hybridization signals in neurons, cortical thymocytes, ganglia, ovarian granulosa cells, testis, and kidney, and detectable protein levels in lung, thymocytes, mouse leukocytes and vascular endothelial cells (2, 7). CD99-L2 expression on endothelial cells is reported to mediate cell aggregation and neutrophil or monocyte extravasation to inflamed tissue in vivo, while CD99 mediates lymphocyte extravasation as well (3, 7).

Antibodies; Abs to CD Markers

Western Blot (WB)

Suitable for use in Western Blot.
Dilution: Western Blot: 0.1-0.2ug/ml with the appropriate secondary reagents to detect mouse CD99-L2. The detection limit for rmCD99-L2 is approximately 2ng/lane under non-reducing and reducing
conditions.

18,848 Da[Similar Products]

CD99 molecule

CD99 antigen; E2 antigen; OTTHUMP00000022840; OTTHUMP00000022842; OTTHUMP00000022843; OTTHUMP00000022844; surface antigen MIC2; T-cell surface glycoprotein E2; MIC2 (monoclonal antibody 12E7); antigen identified by monoclonal 12E7, Y homolog; antigen identified by monoclonal antibodies 12E7, F21 and O13

CD99 antigen

MIC2; MIC2X; MIC2Y  [Similar Products]

CD99_HUMAN

The protein encoded by this gene is a cell surface glycoprotein involved in leukocyte migration, T-cell adhesion, ganglioside GM1 and transmembrane protein transport, and T-cell death by a caspase-independent pathway. In addition, the encoded protein may have the ability to rearrange the actin cytoskeleton and may also act as an oncosuppressor in osteosarcoma. Cyclophilin A binds to CD99 and may act as a signaling regulator of CD99. This gene is found in the pseudoautosomal region of chromosomes X and Y and escapes X-chromosome inactivation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

CD99: Involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. Plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1. Involved in T-cell adhesion processes. Belongs to the CD99 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: Xp22.32 and Yp11.3

Cellular Component: focal adhesion; integral to plasma membrane; cytoplasm; plasma membrane

Biological Process: cell adhesion

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