ATP Binding Cassette Transporter B4, Polyclonal Antibody

ABC-B4; ABC21; MDR2/3; MDR3; PFIC-3; PGY3; GBD1; Multidrug resistance protein 3; P-glycoprotein 3

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

IgG

Rabbit

The antibody is a rabbit polyclonal antibody raised against ABCB4. It has been selected for its ability to recognize ABCB4 in immunohistochemical staining and western blotting.

Affinity Chromatography

Supplied as solution form in PBS, pH7.4, Containing 0.02% NaN3, 50% glycerol.

200ug/ml (lot specific)

Store at 4 degree C for frequent use. Stored at -20 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.

Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.

Small volumes of anti-ABCB4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)

Western blotting: 1:50-400
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-1:5000

Western Blot: Sample: Recombinant protein.

DAB staining on fromalin fixed paraffin- embedded liver tissue)

140,377 Da

ATP-binding cassette, sub-family B (MDR/TAP), member 4

phosphatidylcholine translocator ABCB4

Phosphatidylcholine translocator ABCB4

ABCB4: a transporter that mediates ATP-dependent export of organic anions and drugs from the cytoplasm. Hydrolyzes ATP with low efficiency. Human ABCB4 is not capable of conferring drug resistance. Mediates the translocation of phosphatidylcholine across the canalicular membrane of the hepatocyte. Interacts with HAX1. Defects are the cause of gallstones, and progressive familial intrahepatic cholestasis 3, an autosomal recessive liver disorder that progresses to cirrhosis and liver failure before *****hood. Two alternatively spliced human isoforms have been described.

Protein type: EC 3.6.3.44; Hydrolase; Membrane protein, integral; Membrane protein, multi-pass; Transporter; Transporter, ABC family

Chromosomal Location of Human Ortholog: 5 A1|5 3.43 cM

Cellular Component: actin cytoskeleton; apical plasma membrane; cytoplasm; cytoplasmic vesicle; cytosol; extracellular exosome; focal adhesion; Golgi membrane; integral component of membrane; intercellular canaliculus; membrane; nucleoplasm; plasma membrane

Molecular Function: ATP binding; ATPase activity; ATPase activity, coupled to transmembrane movement of substances; hydrolase activity; nucleotide binding; phosphatidylcholine transmembrane transporter activity; phosphatidylcholine-translocating ATPase activity; xenobiotic transmembrane transporting ATPase activity

Biological Process: bile acid secretion; drug transmembrane transport; lipid homeostasis; lipid transport; phospholipid translocation; positive regulation of cholesterol transport; positive regulation of phospholipid transport; response to fenofibrate; transmembrane transport; transport

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