Full Product Name
PHF6 Antibody (Center)
Product Synonym Names
Purified Rabbit Polyclonal Antibody (Pab); PHD finger protein 6; PHD-like zinc finger protein; PHF6; CENP-31; KIAA1823
Product Gene Name
anti-PHF6 antibody
[Similar Products]
Antibody/Peptide Pairs
PHF6 peptide (MBS9230566) is used for blocking the activity of PHF6 antibody (MBS9216942)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q8IWS0
Antigen Type
Synthesized Peptide
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Other Notes
Small volumes of anti-PHF6 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-PHF6 antibody
Transcriptional regulator that associates with ribosomal RNA promoters and suppresses ribosomal RNA (rRNA) transcription.
Applications Tested/Suitable for anti-PHF6 antibody
Western Blot (WB)
Application Notes for anti-PHF6 antibody
WB~~1:2000
Western Blot (WB) of anti-PHF6 antibody
All lanes : Anti-PHF6 Antibody (Center) at 1:2000 dilution
Lane 1: 293T/17 whole cell lysate
Lane 2: Jurkat whole cell lysate
Lane 3: K562 whole cell lysate
Lysates/proteins at 20 ug per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 41 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
NCBI/Uniprot data below describe general gene information for PHF6. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001015877.1
[Other Products]
NCBI GenBank Nucleotide #
NM_001015877.1
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UniProt Primary Accession #
Q8IWS0
[Other Products]
UniProt Secondary Accession #
Q5JRC7; Q5JRC8; Q96JK3; Q9BRU0; A8K230; B4E0G4; D3DTG3; E9PC97[Other Products]
UniProt Related Accession #
Q8IWS0[Other Products]
Molecular Weight
37,628 Da
NCBI Official Full Name
PHD finger protein 6 isoform 1
NCBI Official Synonym Full Names
PHD finger protein 6
NCBI Official Symbol
PHF6 [Similar Products]
NCBI Official Synonym Symbols
BFLS; BORJ; CENP-31
[Similar Products]
NCBI Protein Information
PHD finger protein 6
UniProt Protein Name
PHD finger protein 6
UniProt Synonym Protein Names
PHD-like zinc finger protein
Protein Family
PHD finger protein
UniProt Gene Name
PHF6 [Similar Products]
UniProt Synonym Gene Names
CENP-31; KIAA1823 [Similar Products]
UniProt Entry Name
PHF6_HUMAN
NCBI Summary for PHF6
This gene is a member of the plant homeodomain (PHD)-like finger (PHF) family. It encodes a protein with two PHD-type zinc finger domains, indicating a potential role in transcriptional regulation, that localizes to the nucleolus. Mutations affecting the coding region of this gene or the splicing of the transcript have been associated with Borjeson-Forssman-Lehmann syndrome (BFLS), a disorder characterized by mental retardation, epilepsy, hypogonadism, hypometabolism, obesity, swelling of subcutaneous tissue of the face, narrow palpebral fissures, and large ears. Alternate splicing results in multiple transcript variants, encoding different isoforms. [provided by RefSeq, Jun 2010]
UniProt Comments for PHF6
PHF6: May play a role in transcriptional regulation. Defects in PHF6 are the cause of Boerjeson-Forssman- Lehmann syndrome (BFLS); also known as Boerjeson- Forssman syndrome (BORJ). BFLS is a X-linked recessive disorder characterized by moderate to severe mental retardation, epilepsy, hypogonadism, hypometabolism, obesity with marked gynecomastia, swelling of subcutaneous tissue of the face, narrow palpebral fissure and large but not deformed ears. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Unknown function; Nucleolus
Chromosomal Location of Human Ortholog: Xq26.3
Cellular Component: nucleolus; nucleoplasm; nucleus
Molecular Function: DNA binding; enzyme binding; histone binding; histone deacetylase binding; phosphoprotein binding; protein binding; ribonucleoprotein binding; tubulin binding; zinc ion binding
Biological Process: regulation of transcription, DNA-dependent; transcription, DNA-dependent
Disease: Borjeson-forssman-lehmann Syndrome
Product References and Citations for anti-PHF6 antibody
Lower K.M.,et al.Nat. Genet. 32:661-665(2002). Nagase T.,et al.DNA Res. 8:85-95(2001). Ota T.,et al.Nat. Genet. 36:40-45(2004). Ross M.T.,et al.Nature 434:325-337(2005). Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
Research Articles on PHF6
1. Female phenotypes of Borjeson-Forssman-Lehmann syndrome patients with PHF6 mutations
Precautions
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Disclaimer
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