Lamp2, Recombinant Protein

Lysosome-associated membrane glycoprotein 2; Lamp2; CD107b; Lamp II; Lamp-2; Lamp-2a; Lamp-2b; Lamp-2c; LGP-B; Mac3

For Research Use Only. Not for use in diagnostic procedures.

Baculovirus

> 95% by SDS-PAGE

Liquid. In phosphate buffered saline (pH 7.4) containing 10% glycerol.

0.5 mg/ml (determined by Absorbance at 280nm) (lot specific)

Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.

Small volumes of Lamp2 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Lamp2, also known as lysosome-associated membrane glycoprotein 2, is a major component of lysosomal membranes. It is clinically characterized by cardiomyopathy, myopathy, and variable mental retardation. Up-regulated Lamp2 at the plasma membrane serves as an indicator of cell activation of CD8+ T cells, mast cells, monocytes, and platelets. Recombinant mouse Lamp2, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Proteases & Inhibitors

SDS-PAGE

40.2kDa (362aa) 50-70KDa (SDS-PAGE under reducing conditions)

lysosomal-associated membrane protein 2

lysosome-associated membrane glycoprotein 2

Lysosome-associated membrane glycoprotein 2

Lamp-2; LAMP-2; Lysosome-associated membrane protein 2; LGP-B  [Similar Products]

Plays an important role in chaperone-mediated autophagy, a process that mediates lysosomal degradation of proteins in response to various stresses and as part of the normal turnover of proteins with a long biological half-live (PubMed:10972293). Functions by binding target proteins, such as GAPDH and MLLT11, and targeting them for lysosomal degradation (). Required for the fusion of autophagosomes with lysosomes during autophagy (PubMed:27628032). Cells that lack LAMP2 express normal levels of VAMP8, but fail to accumulate STX17 on autophagosomes, which is the most likely explanation for the lack of fusion between autophagosomes and lysosomes (PubMed:27628032). Required for normal degradation of the contents of autophagosomes (PubMed:10972293, PubMed:12221139). Plays a role in lysosomal protein degradation in response to starvation (PubMed:27628032). Required for efficient MHCII-mediated presentation of exogenous antigens via its function in lysosomal protein degradation; antigenic peptides generated by proteases in the endosomal/lysosomal compartment are captured by nascent MHCII subunits. Is not required for efficient MHCII-mediated presentation of endogenous antigens ().

Eskelinen EL., et al. (2003) Trends Cell Biol. 13:137-145.; Bertini E., et al. (2005) Neuropediatrics. 36:309-313.

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