Product Name
MBTPS2, siRNA
Full Product Name
MBTPS2 siRNA (Mouse)
Product Synonym Names
Membrane-bound transcription factor site-2 protease; Endopeptidase S2P
Product Gene Name
MBTPS2 sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q8CHX6
Specificity
MBTPS2 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse MBTPS2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of MBTPS2 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
MBTPS2 sirna
siRNA to inhibit MBTPS2 expression using RNA interference
Applications Tested/Suitable for MBTPS2 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for MBTPS2. It may not necessarily be applicable to this product.
NCBI Accession #
NP_758511.1
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NCBI GenBank Nucleotide #
NM_172307.3
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UniProt Primary Accession #
Q8CHX6
[Other Products]
UniProt Secondary Accession #
A2AC85[Other Products]
UniProt Related Accession #
Q8CHX6[Other Products]
Molecular Weight
56,960 Da
NCBI Official Full Name
membrane-bound transcription factor site-2 protease
NCBI Official Synonym Full Names
membrane-bound transcription factor peptidase, site 2
NCBI Official Symbol
Mbtps2 [Similar Products]
NCBI Official Synonym Symbols
AI662535; 9630032G22Rik
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NCBI Protein Information
membrane-bound transcription factor site-2 protease
UniProt Protein Name
Membrane-bound transcription factor site-2 protease
UniProt Synonym Protein Names
Endopeptidase S2P
Protein Family
Membrane-bound transcription factor site-2 protease
UniProt Gene Name
Mbtps2 [Similar Products]
UniProt Entry Name
MBTP2_MOUSE
UniProt Comments for MBTPS2
MBTPS2: Intramembrane proteolysis of sterol-regulatory element- binding proteins (SREBPs) within the first transmembrane segment thereby releasing the N-terminal segment with a portion of the transmembrane segment attached. Site-2 cleavage comes after site-1 cleavage which takes place in the lumenal loop. Expressed in heart, brain, placenta, lung, liver, muscle, kidney and pancreas. Belongs to the peptidase M50A family.
Protein type: Membrane protein, integral; EC 3.4.24.85; Protease; Membrane protein, multi-pass
Cellular Component: cytoplasm
Biological Process: membrane protein intracellular domain proteolysis; positive regulation of transcription factor activity
Research Articles on MBTPS2
1. Data detected yy1, yy2, and mbtps2 mRNA in primary mouse neurons, microglia cells, and astrocytes. In comparison to yy2 and mbtps2, yy1 revealed the highest expression level in all cell types.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
