Full Product Name
NAB2 Polyclonal Antibody
Product Synonym Names
MADER
Product Gene Name
anti-NAB2 antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q15742
Species Reactivity
Human, Mouse, Rat
Purity/Purification
Affinity Purification
Concentration
1mg/ml (lot specific)
Immunogen
Recombinant Protein
Immunogen
Recombinant protein of human NAB2
Calculated Molecular Weight
57kDa
Preparation and Storage
Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Other Notes
Small volumes of anti-NAB2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-NAB2 antibody
This gene encodes a member of the family of NGFI-A binding (NAB) proteins, which function in the nucleus to repress transcription induced by some members of the EGR (early growth response) family of transactivators. NAB proteins can homo- or hetero-multimerize with other EGR or NAB proteins through a conserved N-terminal domain, and repress transcription through two partially redundant C-terminal domains. Transcriptional repression by the encoded protein is mediated in part by interactions with the nucleosome remodeling and deactylase (NuRD) complex. Alternatively spliced transcript variants have been described, but their
biological validity has not been determined.
Product Categories/Family for anti-NAB2 antibody
Polyclonal
Applications Tested/Suitable for anti-NAB2 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-NAB2 antibody
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
Western Blot (WB) of anti-NAB2 antibody
Western blot analysis of extracts of various cell lines, using NAB2 antibody.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
NCBI/Uniprot data below describe general gene information for NAB2. It may not necessarily be applicable to this product.
NCBI Accession #
Q15742.1
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UniProt Primary Accession #
Q15742
[Other Products]
UniProt Secondary Accession #
O76006; Q14797; B2RAK3[Other Products]
UniProt Related Accession #
Q15742[Other Products]
NCBI Official Full Name
NGFI-A-binding protein 2
NCBI Official Synonym Full Names
NGFI-A binding protein 2 (EGR1 binding protein 2)
NCBI Official Symbol
NAB2 [Similar Products]
NCBI Official Synonym Symbols
MADER
[Similar Products]
NCBI Protein Information
NGFI-A-binding protein 2; EGR1 binding protein 2; EGR-1-binding protein 2; melanoma-associated delayed early response protein
UniProt Protein Name
NGFI-A-binding protein 2
UniProt Synonym Protein Names
EGR-1-binding protein 2; Melanoma-associated delayed early response protein; Protein MADER
Protein Family
NGFI-A-binding protein
UniProt Gene Name
NAB2 [Similar Products]
UniProt Synonym Gene Names
MADER; Protein MADER [Similar Products]
UniProt Entry Name
NAB2_HUMAN
NCBI Summary for NAB2
This gene encodes a member of the family of NGFI-A binding (NAB) proteins, which function in the nucleus to repress transcription induced by some members of the EGR (early growth response) family of transactivators. NAB proteins can homo- or hetero-multimerize with other EGR or NAB proteins through a conserved N-terminal domain, and repress transcription through two partially redundant C-terminal domains. Transcriptional repression by the encoded protein is mediated in part by interactions with the nucleosome remodeling and deactylase (NuRD) complex. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]
UniProt Comments for NAB2
NAB2: Acts as a transcriptional repressor for zinc finger transcription factors EGR1 and EGR2. Isoform 2 lacks repression ability. Belongs to the NAB family. 3 isoforms of the human protein are produced by alternative splicing.
Chromosomal Location of Human Ortholog: 12q13.3
Cellular Component: nucleus
Molecular Function: transcription corepressor activity; transcription factor binding
Biological Process: Schwann cell differentiation; myelination; nervous system development; cell proliferation; transcription, DNA-dependent; negative regulation of transcription from RNA polymerase III promoter; regulation of epidermis development; endochondral ossification
Research Articles on NAB2
1. Tumors with the most common fusion variant, NAB2ex4-STAT6ex2/3, corresponded to classic pleuropulmonary solitary fibrous tumors with diffuse fibrosis and mostly benign behavior and occurred in older patients
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