Full Product Name
AGXT siRNA (Mouse)
Product Synonym Names
AGXT1; Serine--pyruvate aminotransferase mitochondrial; SPT; Alanine--glyoxylate aminotransferase; AGT
Product Gene Name
AGXT sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for O35423
Specificity
AGXT siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse AGXT gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of AGXT sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
AGXT sirna
siRNA to inhibit AGXT expression using RNA interference
Applications Tested/Suitable for AGXT sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for AGXT. It may not necessarily be applicable to this product.
NCBI Accession #
NP_057911.2
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NCBI GenBank Nucleotide #
NM_016702.3
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UniProt Primary Accession #
O35423
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UniProt Secondary Accession #
Q8R128[Other Products]
UniProt Related Accession #
O35423[Other Products]
Molecular Weight
43,520 Da
NCBI Official Full Name
serine--pyruvate aminotransferase, mitochondrial isoform 1
NCBI Official Synonym Full Names
alanine-glyoxylate aminotransferase
NCBI Official Symbol
Agxt [Similar Products]
NCBI Official Synonym Symbols
AGT; SPT; Agt1; Agxt1
[Similar Products]
NCBI Protein Information
serine--pyruvate aminotransferase, mitochondrial; serine--pyruvate aminotransferase, peroxisomal
UniProt Protein Name
Serine--pyruvate aminotransferase, mitochondrial
UniProt Synonym Protein Names
Alanine--glyoxylate aminotransferase (EC:2.6.1.44); AGT
Protein Family
Serine--pyruvate aminotransferase
UniProt Gene Name
Agxt [Similar Products]
UniProt Synonym Gene Names
Agxt1; SPT; AGT [Similar Products]
UniProt Entry Name
SPYA_MOUSE
UniProt Comments for AGXT
AGXT: Defects in AGXT are the cause of hyperoxaluria primary type 1 (HP1); also known as primary hyperoxaluria type I (PH1) and oxalosis I. HP1 is a rare autosomal recessive inborn error of glyoxylate metabolism characterized by increased excretion of oxalate and glycolate, and the progressive accumulation of insoluble calcium oxalate in the kidney and urinary tract. Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family.
Protein type: Transferase; Amino Acid Metabolism - glycine, serine and threonine; Amino Acid Metabolism - alanine, aspartate and glutamate; Mitochondrial; EC 2.6.1.51; EC 2.6.1.44; Motility/polarity/chemotaxis
Cellular Component: peroxisomal matrix; mitochondrion; mitochondrial matrix; peroxisome
Molecular Function: transferase activity; amino acid binding; protein homodimerization activity; alanine-glyoxylate transaminase activity; serine-pyruvate transaminase activity; transaminase activity; catalytic activity; pyridoxal phosphate binding; receptor binding
Biological Process: pyruvate biosynthetic process; response to cAMP; glyoxylate catabolic process; metabolic process; glyoxylate metabolic process; glycine biosynthetic process, by transamination of glyoxylate; response to glucocorticoid stimulus; oxalic acid secretion; L-cysteine catabolic process; L-alanine catabolic process
Research Articles on AGXT
1. expressed wild-type human AGT1 was predominantly localized in mouse hepatocellular peroxisomes, whereas the most common mutant form of AGT1 (G170R) was localized predominantly in the mitochondria
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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