AGXT, Polyclonal Antibody

Serine--pyruvate aminotransferase; SPT; Alanine--glyoxylate aminotransferase; AGT; AGXT; AGT1; SPAT

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit Ig

Rabbit

This AGXT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 96-125 amino acids from the Central region of human AGXT.

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Vial Concentration: 1.09 (lot specific)

Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Small volumes of anti-AGXT antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

AGXT is expressed only in the liver and protein is localized mostly in the peroxisomes, where it is involved in glyoxylate detoxification.

Metabolism; Signal Transduction

Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)

WB~~1:1000

Anti-AGXT Antibody (Center)at 1:2000 dilution + human liver lysates

Lysates/proteins at 20 ug per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution

Predicted band size : 43 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

Anti-AGXT Antibody (Center)at 1:2000 dilution + human liver lysates

Lysates/proteins at 20 ug per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution

Predicted band size : 43 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

Anti-AGXT Antibody (Center)at 1:2000 dilution + human liver lysates

Lysates/proteins at 20 ug per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution

Predicted band size : 43 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

Western blot analysis of AGXT Antibody (Center) in HepG2 cell line lysates (35ug/lane). AGXT (arrow) was detected using the purified Pab.(8ug/ml)

AGXT Antibody (Center) (RB18848) IHC analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the AGXT Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

AGXT Antibody (Center) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

43010

alanine-glyoxylate aminotransferase

serine--pyruvate aminotransferase

Serine--pyruvate aminotransferase

AGT1; SPAT; SPT; AGT  [Similar Products]

SPYA_HUMAN

This gene is expressed only in the liver and the encoded protein is localized mostly in the peroxisomes, where it is involved in glyoxylate detoxification. Mutations in this gene, some of which alter subcellular targetting, have been associated with type I primary hyperoxaluria. [provided by RefSeq, Jul 2008]

AGXT: Defects in AGXT are the cause of hyperoxaluria primary type 1 (HP1); also known as primary hyperoxaluria type I (PH1) and oxalosis I. HP1 is a rare autosomal recessive inborn error of glyoxylate metabolism characterized by increased excretion of oxalate and glycolate, and the progressive accumulation of insoluble calcium oxalate in the kidney and urinary tract. Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family.

Protein type: Mitochondrial; EC 2.6.1.44; Amino Acid Metabolism - alanine, aspartate and glutamate; Motility/polarity/chemotaxis; Transferase; Amino Acid Metabolism - glycine, serine and threonine; EC 2.6.1.51

Chromosomal Location of Human Ortholog: 2q37.3

Cellular Component: peroxisomal matrix; mitochondrial matrix; peroxisome

Molecular Function: amino acid binding; protein binding; protein homodimerization activity; alanine-glyoxylate transaminase activity; serine-pyruvate transaminase activity; transaminase activity; pyridoxal phosphate binding; receptor binding

Biological Process: pyruvate biosynthetic process; response to cAMP; glyoxylate catabolic process; glyoxylate metabolic process; glycine biosynthetic process, by transamination of glyoxylate; response to glucocorticoid stimulus; oxalic acid secretion; L-cysteine catabolic process; L-alanine catabolic process

Disease: Hyperoxaluria, Primary, Type I

Cellini,B., J. Biol. Chem. 284 (13), 8349-8358 (2009)
Bertoldi,M., Biochim. Biophys. Acta 1784 (9), 1356-1362 (2008)

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