Alanine Glyoxylate Aminotransferase, Polyclonal Antibody

AGT; AGT1; AGXT1; PH1; SPAT; SPT; TLH6; Oxalosis I; Hyperoxaluria I; Glycolicaciduria; Serine-Pyruvate Aminotransferase

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

IgG

Rabbit

The antibody is a rabbit polyclonal antibody raised against AGXT. It has been selected for its ability to recognize AGXT in immunohistochemical staining and western blotting.

Affinity Chromatography

Supplied as solution form in PBS, pH7.4 containing 0.02% NaN3, 50% glycerol.

200ug/ml (lot specific)

Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.

Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.

Small volumes of anti-AGXT antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)

Western Blot: 1:50-400
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-200

Western Blot: Sample: Recombinant protein.

DAB staining on fromalin fixed paraffin- embedded liver tissue)

DAB staining on IHC-P; Samples: Mouse Liver Tissue)

43,520 Da

alanine-glyoxylate aminotransferase

serine--pyruvate aminotransferase, mitochondrial; serine--pyruvate aminotransferase, peroxisomal

Serine--pyruvate aminotransferase, mitochondrial

Agxt1; SPT; AGT  [Similar Products]

AGXT: Defects in AGXT are the cause of hyperoxaluria primary type 1 (HP1); also known as primary hyperoxaluria type I (PH1) and oxalosis I. HP1 is a rare autosomal recessive inborn error of glyoxylate metabolism characterized by increased excretion of oxalate and glycolate, and the progressive accumulation of insoluble calcium oxalate in the kidney and urinary tract. Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family.

Protein type: Amino Acid Metabolism - alanine, aspartate and glutamate; Amino Acid Metabolism - glycine, serine and threonine; EC 2.6.1.44; EC 2.6.1.51; Mitochondrial; Motility/polarity/chemotaxis; Transferase

Chromosomal Location of Human Ortholog: 1 D|1 47.0 cM

Cellular Component: intracellular membrane-bound organelle; mitochondrial matrix; mitochondrion; peroxisomal matrix; peroxisome

Molecular Function: alanine-glyoxylate transaminase activity; amino acid binding; catalytic activity; protein homodimerization activity; protein self-association; pyridoxal phosphate binding; receptor binding; serine-pyruvate transaminase activity; transaminase activity; transferase activity

Biological Process: glycine biosynthetic process, by transamination of glyoxylate; glyoxylate catabolic process; glyoxylate metabolic process; L-alanine catabolic process; L-cysteine catabolic process; Notch signaling pathway; oxalic acid secretion; pyruvate biosynthetic process; response to cAMP; response to glucocorticoid stimulus

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