Product Name
FOXP2, siRNA
Full Product Name
FOXP2 siRNA (Human)
Product Synonym Names
CAGH44; TNRC10; Forkhead box protein P2; CAG repeat protein 44; Trinucleotide repeat-containing gene 10 protein
Product Gene Name
FOXP2 sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for O15409
Specificity
FOXP2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human FOXP2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of FOXP2 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
FOXP2 sirna
siRNA to inhibit FOXP2 expression using RNA interference
Applications Tested/Suitable for FOXP2 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for FOXP2. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001166237.1
[Other Products]
NCBI GenBank Nucleotide #
NM_001172766.2
[Other Products]
UniProt Primary Accession #
O15409
[Other Products]
UniProt Secondary Accession #
Q6ZND1; Q75MJ3; Q8IZE0; Q8N0W2; Q8N6B7; Q8N6B8; Q8NFQ1; A0AUV6; A4D0U8; A6NNW4; B4DLD9[Other Products]
UniProt Related Accession #
O15409[Other Products]
Molecular Weight
81,840 Da
NCBI Official Full Name
forkhead box protein P2 isoform V
NCBI Official Synonym Full Names
forkhead box P2
NCBI Official Symbol
FOXP2 [Similar Products]
NCBI Official Synonym Symbols
SPCH1; CAGH44; TNRC10
[Similar Products]
NCBI Protein Information
forkhead box protein P2
UniProt Protein Name
Forkhead box protein P2
UniProt Synonym Protein Names
CAG repeat protein 44; Trinucleotide repeat-containing gene 10 protein
Protein Family
Forkhead box protein
UniProt Gene Name
FOXP2 [Similar Products]
UniProt Synonym Gene Names
CAGH44; TNRC10 [Similar Products]
UniProt Entry Name
FOXP2_HUMAN
NCBI Summary for FOXP2
This gene encodes a member of the forkhead/winged-helix (FOX) family of transcription factors. It is expressed in fetal and ***** brain as well as in several other organs such as the lung and gut. The protein product contains a FOX DNA-binding domain and a large polyglutamine tract and is an evolutionarily conserved transcription factor, which may bind directly to approximately 300 to 400 gene promoters in the human genome to regulate the expression of a variety of genes. This gene is required for proper development of speech and language regions of the brain during embryogenesis, and may be involved in a variety of
biological pathways and cascades that may ultimately influence language development. Mutations in this gene cause speech-language disorder 1 (SPCH1), also known as autosomal dominant speech and language disorder with orofacial dyspraxia. Multiple alternative transcripts encoding different isoforms have been identified in this gene.[provided by RefSeq, Feb 2010]
UniProt Comments for FOXP2
FOXP2: Transcriptional repressor that may play a role in the specification and differentiation of lung epithelium. May also play a role in developing neural, gastrointestinal and cardiovascular tissues. Can act with CTBP1 to synergistically repress transcription but CTPBP1 is not essential. Involved in neural mechanisms mediating the development of speech and language. Forms homodimers and heterodimers with FOXP1 and FOXP4. Dimerization is required for DNA-binding. Interacts with CTBP1. Isoform 1 and isoform 6 are expressed in ***** and fetal brain, caudate nucleus and lung. 9 isoforms of the human protein are produced by alternative splicing.
Protein type: DNA-binding; C2H2-type zinc finger protein
Chromosomal Location of Human Ortholog: 7q31
Cellular Component: nucleus
Molecular Function: protein binding; protein homodimerization activity; DNA binding; sequence-specific DNA binding; protein heterodimerization activity; metal ion binding; transcription factor activity
Biological Process: caudate nucleus development; skeletal muscle development; camera-type eye development; transcription, DNA-dependent; righting reflex; putamen development; negative regulation of transcription from RNA polymerase II promoter; vocal learning; post-embryonic development; smooth muscle development; positive regulation of mesenchymal cell proliferation; cerebellum development; cerebral cortex development; negative regulation of transcription, DNA-dependent; alveolus development; growth
Disease: Speech-language Disorder 1
Research Articles on FOXP2
1. FOXP2 SNPs influence the use of speech sound learning strategies.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
