DAP5, Monoclonal Antibody

Anti -DAP5 (Death Associated Protein 5, EIF4g2, Eukaryotic Elongation Factor 4 gamma 2)

For Research Use Only. Not for use in diagnostic procedures.

Chromosome: 11; NC_000011.9 (10818593..10830582, complement). Location: 11p15

Monoclonal

IgG

8J1261

Mouse

In Jurkat, a 97kD band is observed. Species Crossreactivity: Crossreacts with Human and Mouse. Predicted to crossreact with Rat, Bovine, Canine and Monkey proteins based on 100% identity of the immunogen.

Affinity Purified
Purified by immunoaffinity chromatography.

PBS containing 0.2% gelatin, 0.05% sodium azide.

May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Small volumes of anti-EIF4G2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Recently, five novel apoptotic genes (named DAP genes for: Death Associated Proteins) have been identified that code for proteins, which display a diverse spectrum of biochemical activities. These include a novel type of calcium/calmodulin-regulated kinase, which carries ankyrin repeats and a death domain (DAP-kinase), a nucleotide-binding protein (DAP-3), a small proline-rich cytoplasmic protein (DAP-1), and a novel homolog of the eIF4G translation initiation factor (DAP-5) (1-2). DAP-5 (also named p97 and NAT1) is a ubiquitously expressed member of the translation initiation factor eIF4G family that lacks the eIF4E . DAP-5 was cloned from HeLa cells using a functional approach in attempt to isolate cDNA fragments, which convey resistance to gamma interferon (IFN-g)-induced programmed cell death (3). A fragment of the cDNA when expressed as a mini-protein of 28kD protects the cell from IFN-g induced apoptosis. The full-length DAP-5 cDNA codes for a protein of approximate molecular weight of 97kD. Low level of expression protects the cells, whereas high level of expression results in cell death (3-4).

Antibodies; Abs to Apoptosis

Western Blot (WB)

Suitable for use in Western Blot.

102,362 Da[Similar Products]

eukaryotic translation initiation factor 4 gamma, 2

eukaryotic translation initiation factor 4 gamma 2; DAP-5; eIF4G 2; eIF-4G 2; eIF-4-gamma 2; OTTHUMP00000231076; OTTHUMP00000231227; OTTHUMP00000231228; OTTHUMP00000231229; aging-associated protein 1; death-associated protein 5; eukaryotic translation initiation factor 4G-like 1

Eukaryotic translation initiation factor 4 gamma 2

DAP5  [Similar Products]

IF4G2_HUMAN

Translation initiation is mediated by specific recognition of the cap structure by eukaryotic translation initiation factor 4F (eIF4F), which is a cap binding protein complex that consists of three subunits: eIF4A, eIF4E and eIF4G. The protein encoded by this gene shares similarity with the C-terminal region of eIF4G that contains the s for eIF4A and eIF3; eIF4G, in addition, contains a for eIF4E at the N-terminus. Unlike eIF4G, which supports cap-dependent and independent translation, this gene product functions as a general repressor of translation by forming translationally inactive complexes. In vitro and in vivo studies indicate that translation of this mRNA initiates exclusively at a non-AUG (GUG) codon. Alternatively spliced transcript variants encoding different isoforms of this gene have been described. [provided by RefSeq]

EIF4G2: a general repressor of translation by forming translationally inactive complexes. Appears to play a role in the switch from cap-dependent to IRES-mediated translation during mitosis, apoptosis and viral infection. Interacts with the protein kinases Mnk1 and -2. Binds EIF4A and EIF3. Cleaved by some caspases and viral proteases.

Protein type: Translation initiation; Translation; RNA-binding

Chromosomal Location of Human Ortholog: 11p15

Cellular Component: membrane; eukaryotic translation initiation factor 4F complex; cytosol

Molecular Function: protein binding; translation factor activity, nucleic acid binding; translation initiation factor activity

Biological Process: cell death; cytokine and chemokine mediated signaling pathway; translational initiation; cell cycle arrest; regulation of translational initiation

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