Product Name
Tim3 (HAVCR2), Monoclonal Antibody
Popular Item
Full Product Name
Tim3 (T Cell Immunoglobulin and Mucin Domain-containing 3, T Cell Immunoglobulin Mucin 3, TIM3, TIM-3, TIMD3, FLJ14428, Hepatitis A Virus Cellular Receptor 2, HAVCR 2, HAVCR2, HAVCR-2, Kidney Injury Molecule 3, KIM-3) (APC)
Product Synonym Names
Anti -Tim3 (T Cell Immunoglobulin and Mucin Domain-containing 3, T Cell Immunoglobulin Mucin 3, TIM3, TIM-3, TIMD3, FLJ14428, Hepatitis A Virus Cellular Receptor 2, HAVCR 2, HAVCR2, HAVCR-2, Kidney Injury Molecule 3, KIM-3) (APC)
Product Gene Name
anti-HAVCR2 antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
chromosome: 5; Location: 5q33.3
3D Structure
ModBase 3D Structure for Q8TDQ0
Specificity
Recognizes human Tim3.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Form/Format
Supplied as a liquid in saline, 0.5% BSA, 0.09% sodium azide. Labeled with Allophycocyanin (APC)
Concentration
0.01 mg/ml (lot specific)
Immunogen
Recombinant protein corresponding to aa22-200 from the extracellular domain of human Tim3 expressed in NSO cells (NP_116171).
Preparation and Storage
May be stored at 4 degree C before opening. DO NOT FREEZE! Stable at 4 degree C as an undiluted liquid. Stable for at 12 months at 4 degree C. Freezing allophycocyanin conjugates will result in a substantial loss of activity. Allophycocyanin conjugates are sensitive to light.
Other Notes
Small volumes of anti-HAVCR2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-HAVCR2 antibody
T cell immunoglobulin mucin-3 (Tim3) is a member of the T cell Immunoglobulin- and Mucin-domain-containing family of type I membrane glycoproteins that regulate autoimmune and allergic disease. Tim3 is selectively expressed on Th1 cells and interacts with galectin-9. It negatively regulates Th1 responses and affects macrophage activation. The 280aa mature human Tim3 contains a V-type Ig-like domain that shows multiple polymorphisms, followed by a mucin-like domain in the 171aa extracellular region, which shares 60% aa identity with the mouse Tim3 ECD. One splice variant is truncated within the mucin domain and presumably is secreted.
Product Categories/Family for anti-HAVCR2 antibody
Antibodies; Abs to T Cells
Applications Tested/Suitable for anti-HAVCR2 antibody
Flow Cytometry (FC/FACS)
Application Notes for anti-HAVCR2 antibody
Suitable for use in Flow Cytometry.
Dilution: Flow Cytometry: 10ul labels up to 1x10e6 cells. Validated using whole blood monocytes.
Flow Cytometry Protocol: 1. Cells may be Fc-blocked with 1ug of human IgG/10e5 cells for 15 minutes at RT. Do not wash excess blocking IgG from this reaction.
2. After blocking, add 10ul of T5469-92F to up to 1x10e6 cells and incubate for 30 minutes at RT
3. Remove unbound antibody by washing the cells twice in Flow Cytometry Staining Buffer . Note that whole blood requires a RBC lysis step at this point using Flow Cytometry Human Lyse Buffer.
4. Resuspend the cells in Flow Cytometry Staining Buffer for final flow cytometric analysis. As a control for this analysis, cells in a separate tube should be treated with APC-labeled rat IgG2A antibody.
Flow Cytometry of anti-HAVCR2 antibody
Whole blood monocytes were stained (filled histogram) or isotype control (open histogram)
NCBI/Uniprot data below describe general gene information for HAVCR2. It may not necessarily be applicable to this product.
UniProt Primary Accession #
Q8TDQ0
[Other Products]
UniProt Secondary Accession #
Q8WW60; Q96K94; B2RAY2[Other Products]
UniProt Related Accession #
Q8TDQ0[Other Products]
Molecular Weight
33,394 Da[Similar Products]
NCBI Official Full Name
T cell immunoglobulin mucin-3
NCBI Official Synonym Full Names
hepatitis A virus cellular receptor 2
NCBI Official Symbol
HAVCR2 [Similar Products]
NCBI Official Synonym Symbols
TIM3; KIM-3; TIMD3; Tim-3; FLJ14428; HAVCR2
[Similar Products]
NCBI Protein Information
hepatitis A virus cellular receptor 2; TIMD-3; HAVcr-2; kidney injury molecule-3; T-cell membrane protein 3; T cell immunoglobulin mucin 3; T cell immunoglobulin mucin-3; T-cell immunoglobulin and mucin domain-containing protein 3
UniProt Protein Name
Hepatitis A virus cellular receptor 2
UniProt Synonym Protein Names
T-cell immunoglobulin and mucin domain-containing protein 3; TIMD-3; T-cell membrane protein 3; TIM-3
Protein Family
Hepatitis A virus cellular receptor
UniProt Gene Name
HAVCR2 [Similar Products]
UniProt Synonym Gene Names
TIM3; TIMD3 [Similar Products]
UniProt Entry Name
HAVR2_HUMAN
NCBI Summary for HAVCR2
CD4 (MIM 186940)-positive T helper lymphocytes can be divided into types 1 (Th1) and 2 (Th2) on the basis of their cytokine secretion patterns. Th1 cells and their associated cytokines are involved in cell-mediated immunity to intracellular pathogens and delayed-type hypersensitivity reactions, whereas Th2 cells are involved in the control of extracellular helminthic infections and the promotion of atopic and allergic diseases. The 2 types of cells also cross-regulate the functions of the other. TIM3 is a Th1-specific cell surface protein that regulates macrophage activation and enhances the severity of experimental autoimmune encephalomyelitis in mice.[supplied by OMIM]
UniProt Comments for HAVCR2
TIM-3: Regulates macrophage activation. Inhibits T-helper type 1 lymphocyte (Th1)-mediated auto- and alloimmune responses and promotes immunological tolerance. May be also involved in T-cell homing. Receptor for LGALS9. Belongs to the immunoglobulin superfamily. TIM family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Receptor, misc.; Membrane protein, integral
Chromosomal Location of Human Ortholog: 5q33.3
Cellular Component: integral to membrane
Disease: Rheumatoid Arthritis
Research Articles on HAVCR2
1. expression is increased on Vdelta T cells of pregnant women
2. Mucin domain-containing molecule 3 (Tim-3) is preferentially expressed in lymphoma-derived endothelial cells.
3. Structural and
biologic studies reveal that TIM-3 is a receptor for phosphatidylserine (PtdSer) and that TIM-3 polymorphic variants differ functionally in their recognition of PtdSer and clearance of apoptotic cells.
4. The TIM-3 pathway and the therapeutic potential of modulating the pathway in systemic lupus erythematosus, is reviewed.
5. haplotypes including TIM-3 genetic variants confer susceptibility to asthma in a Chinese population
6. Observational study of gene-disease association, gene-gene interaction, and gene-environment interaction. (HuGE Navigator)
7. Tim-3 expression correlates with a dysfunctional and senescent phenotype (CD127(low) CD57(high)), a central rather than effector memory profile (CD45RA(negative) CCR7(high)), and reduced Th1/Tc1 cytokine production.
8. These results suggest that TIM-3 is a negative regulator of human T cells and regulates Th1 and Th17 cytokine secretion.
9. Reduced TIM-3 expression in the lungs of patients may result in a defective T cell ability to control the Th1 immune response and could thus contribute to the pathogenesis of sarcoidosis.
10. The -1516G>T, -574T>G, and 4259G>T of TIM-3 gene polymorphisms might not play an important role as a genetic risk factor in the pathophysiology of ITP.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
