CD209, Monoclonal Antibody

CD209 antibody

For Research Use Only. Not for use in diagnostic procedures.

Monoclonal

IgG2a kappa

h209

Rat

Purified by affinity chromatography.

Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer.

500 ug/ml (lot specific)

Store at 2-8 degree C. Do not freeze. Protect from light.

Small volumes of anti-CD209 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Immunology

Flow Cytometry (FC/FACS), Immunoprecipitation (IP)

FC: <= 0.5 ug/test

Staining of human monocyte-derived immature dendritic cells with 0.5 ug of Rat IgG2a K Isotype Control (open histogram) or 0.5 ug of CD209 antibody (filled histogram) followed by Anti-Rat IgG (FITC). Cells in the large scatter population were used for analysis.

33,874 Da

CD209 molecule

CD209 antigen

CD209 antigen

CLEC4L; DC-SIGN; DC-SIGN1  [Similar Products]

This gene encodes a transmembrane receptor and is often referred to as DC-SIGN because of its expression on the surface of dendritic cells and macrophages. The encoded protein is involved in the innate immune system and recognizes numerous evolutionarily divergent pathogens ranging from parasites to viruses with a large impact on public health. The protein is organized into three distinct domains: an N-terminal transmembrane domain, a tandem-repeat neck domain and C-type lectin carbohydrate recognition domain. The extracellular region consisting of the C-type lectin and neck domains has a dual function as a pathogen recognition receptor and a cell adhesion receptor by binding carbohydrate ligands on the surface of microbes and endogenous cells. The neck region is important for homo-oligomerization which allows the receptor to bind multivalent ligands with high avidity. Variations in the number of 23 amino acid repeats in the neck domain of this protein are rare but have a significant impact on ligand binding ability. This gene is closely related in terms of both sequence and function to a neighboring gene (GeneID 10332; often referred to as L-SIGN). DC-SIGN and L-SIGN differ in their ligand-binding properties and distribution. Alternative splicing results in multiple variants.[provided by RefSeq, Feb 2009]

CD209: Pathogen-recognition receptor expressed on the surface of immature dendritic cells (DCs) and involved in initiation of primary immune response. Thought to mediate the endocytosis of pathogens which are subsequently degraded in lysosomal compartments. The receptor returns to the cell membrane surface and the pathogen-derived antigens are presented to resting T-cells via MHC class II proteins to initiate the adaptive immune response. Probably recognizes in a calcium-dependent manner high mannose N-linked oligosaccharides in a variety of pathogen antigens, including HIV-1 gp120, HIV-2 gp120, SIV gp120, ebolavirus glycoproteins, cytomegalovirus gB, HCV E2, dengue virus gE, Leishmania pifanoi LPG, Lewis-x antigen in Helicobacter pylori LPS, mannose in Klebsiella pneumonae LPS, di-mannose and tri- mannose in Mycobacterium tuberculosis ManLAM and Lewis-x antigen in Schistosoma mansoni SEA. Homotetramer. Binds to many viral surface glycoproteins such as HIV-1 gp120, HIV-2 gp120, SIV gp120, ebolavirus envelope glycoproteins, cytomegalovirus gB, HCV E2 and dengue virus major envelope protein E. Predominantly expressed in dendritic cells and in DC-residing tissues. Also found in placental macrophages, endothelial cells of placental vascular channels, peripheral blood mononuclear cells, and THP-1 monocytes. 13 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: cell surface; membrane; plasma membrane

Molecular Function: mannose binding; protein binding; virion binding

Biological Process: cell-cell recognition; heterophilic cell adhesion; regulation of T cell proliferation; stimulatory C-type lectin receptor signaling pathway; virion attachment to host cell surface receptor; virus-host interaction

Disease: Human Immunodeficiency Virus Type 1, Susceptibility To; Mycobacterium Tuberculosis, Susceptibility To

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