Diacylglycerol Kinase Gamma (DGKg), Polyclonal Antibody

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

IgG

Rabbit

The antibody is a rabbit polyclonal antibody raised against DGKg. It has been selected for its ability to recognize DGKg in immunohistochemical staining andwestern blotting.

Affinity Chromatography

Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.

200ug/ml (lot specific)

Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.

Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.

Small volumes of anti-DGKg antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)

Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200

Western Blot: Sample: Recombinant protein.

DAB staining on IHC-P; Samples: Rat Kidney Tissue)

88,521 Da

diacylglycerol kinase, gamma

diacylglycerol kinase gamma

Diacylglycerol kinase gamma

Dagk3; DAG kinase gamma; DGK-gamma  [Similar Products]

putative diacylglycerol kinase associated with the cytoskeleton of purkinje cells [RGD, Feb 2006]

DGKG: Reverses the normal flow of glycerolipid biosynthesis by phosphorylating diacylglycerol back to phosphatidic acid. Belongs to the eukaryotic diacylglycerol kinase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.7.1.107; Kinase, lipid; Lipid Metabolism - glycerolipid; Lipid Metabolism - glycerophospholipid; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 11q23

Cellular Component: intracellular

Molecular Function: ATP binding; calcium ion binding; diacylglycerol kinase activity; NAD+ kinase activity

Biological Process: glycerolipid metabolic process; lipid phosphorylation; neuron development; protein kinase C-activating G-protein coupled receptor signaling pathway

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