Product Name
Eosinophil cationic protein (ECP), ELISA Kit
Full Product Name
Monkey Eosinophil cationic protein ELISA Kit
Product Synonym Names
Monkey ECP ELISA Kit; ECP ELISA Kit
Product Gene Name
ECP elisa kit
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Request Current Manual
Chromosome Location
Chromosome: 14; NC_000014.8 (21359562..21360507). Location: 14q24-q31
3D Structure
ModBase 3D Structure for P12724
Species Reactivity
Monkey
Preparation and Storage
Store all reagents at 2-8 degree C
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of ECP elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for ECP purchase
MBS754454 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Eosinophil cationic protein (ECP) ELISA Kit target analytes in
biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ECP. The ELISA analytical biochemical technique of the MBS754454 kit is based on ECP antibody-ECP antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ECP antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ECP. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
NCBI/Uniprot data below describe general gene information for ECP. It may not necessarily be applicable to this product.
NCBI Accession #
NP_002926.2
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NCBI GenBank Nucleotide #
NM_002935.2
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UniProt Primary Accession #
P12724
[Other Products]
UniProt Secondary Accession #
Q4VBC1; Q8WTP7; Q8WZ62; Q9GZN9[Other Products]
UniProt Related Accession #
P12724[Other Products]
Molecular Weight
18,385 Da
NCBI Official Full Name
eosinophil cationic protein
NCBI Official Synonym Full Names
ribonuclease, RNase A family, 3
NCBI Official Symbol
RNASE3 [Similar Products]
NCBI Official Synonym Symbols
ECP; RNS3
[Similar Products]
NCBI Protein Information
eosinophil cationic protein; RNase 3; ribonuclease 3; cytotoxic ribonuclease
UniProt Protein Name
Eosinophil cationic protein
UniProt Synonym Protein Names
Ribonuclease 3
Protein Family
Eosinophil cationic protein
UniProt Gene Name
RNASE3 [Similar Products]
UniProt Synonym Gene Names
ECP; RNS3; ECP; RNase 3 [Similar Products]
UniProt Entry Name
ECP_HUMAN
NCBI Summary for ECP
The protein encoded by this gene belongs to the pancreatic ribonuclease family, a subset of the ribonuclease A superfamily. The protein exhibits antimicrobial activity against pathogenic bacteria [provided by RefSeq, Oct 2014]
UniProt Comments for ECP
RNASE3: Cytotoxin and helminthotoxin with low-efficiency ribonuclease activity. Possesses a wide variety of biological activities. Exhibits antibacterial activity, including cytoplasmic membrane depolarization of preferentially Gram-negative, but also Gram-positive strains. Promotes E.coli outer membrane detachment, alteration of the overall cell shape and partial loss of cell content. Belongs to the pancreatic ribonuclease family.
Protein type: Secreted, signal peptide; Secreted; Ribonuclease; EC 3.1.27.-
Chromosomal Location of Human Ortholog: 14q11.2
Cellular Component: extracellular space; extracellular region
Molecular Function: nucleic acid binding; endonuclease activity; ribonuclease activity
Biological Process: defense response to Gram-positive bacterium; innate immune response in mucosa; RNA catabolic process; antibacterial humoral response
Research Articles on ECP
1. A fraction of native ECP is stored in a non-cytotoxic form, which can be converted into a cytotoxic form by N-deglycosylation, whereas another fraction is stored as a highly cytotoxic form carrying different post-translational modifications
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.