TTF2, Polyclonal Antibody

FKH L15; FKHL 15; FKHL15; Forkhead (Drosophila) like 15; Forkhead box E1; Forkhead box E2; Forkhead box protein E1; Forkhead box protein E2; Forkhead drosophila homolog like 15; Forkhead like 15; Forkhead related protein FKHL 15; Forkhead related protein FKHL15; Forkhead-related protein FKHL15; FOX E1; FOX E2; FOXE 1; FOXE 2; FOXE1; FOXE1_HUMAN; FOXE2; HFKH 4; HFKH4; HFKL 5; HFKL5; HNF-3/fork head-like protein 5; Thyroid transcription factor 2; TITF 2; TITF2; TTF 2; TTF-2; TTF2

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

IgG

Rabbit

TTF2 antibody detects endogenous levels of total TTF2

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

1mg/ml (lot specific)

Store at -20 degree C. Stable for 12 months from date of receipt.

Manufactured in an ISO 9001:2015 Certified Laboratory.

Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available, please inquire.

Small volumes of anti-TTF2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Function: Transcription factor that binds consensus sites on a variety of gene promoters and activate their transcription. Involved in proper palate formation, most probably through the expression of MSX1 and TGFB3 genes which are direct targets of this transcription factor. Also implicated in thyroid gland morphogenesis. May indirectly play a role in cell growth and migration through the regulation of WNT5A expression.
Post-translational Modifications: Phosphorylated.

Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)

WB: 1:1000-3000
IF/ICC: 1:100-1:500

Western blot analysis of TTF2 using 293 whole cell lysates

MBS9604470 staining HuvEc by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.

Observed: 34 kD
Predicted: 39 kDa

forkhead box E1

forkhead box protein E1

Forkhead box protein E1

FKHL15; FOXE2; TITF2; TTF2; HFKL5; TTF-2  [Similar Products]

This intronless gene encodes a protein that belongs to the forkhead family of transcription factors. Members of this family contain a conserved 100-amino acid DNA-binding 'forkhead' domain. The encoded protein functions as a thyroid transcription factor that plays a role in thyroid morphogenesis. Mutations in this gene are associated with the Bamforth-Lazarus syndrome, and with susceptibility to nonmedullary thyroid cancer-4. [provided by RefSeq, Nov 2016]

Transcription factor that binds consensus sites on a variety of gene promoters and activate their transcription. Involved in proper palate formation, most probably through the expression of MSX1 and TGFB3 genes which are direct targets of this transcription factor. Also implicated in thyroid gland morphogenesis. May indirectly play a role in cell growth and migration through the regulation of WNT5A expression.

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