IGF-1R, Polyclonal Antibody

IGFR; CD221; IGFIR; JTK13; kinase IGF1R; Insulin-like growth factor I receptor

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

IgG

Rabbit

Affinity Purified

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

1.0mg/mL (lot specific)

Small volumes of anti-IGF-1R antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Entrez Summary: Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Receptors, Kinase/Phosphatases

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

WB: 1:500~1:1000

Western blot analysis of extracts from 293 cells using IGF-1R (Ab-1161) Antibody and the same antibody preincubated with blocking peptide.

Immunofluorescence staining of methanol-fixed MCF cells using IGF-1R (Ab-1161) Antibody.

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using IGF-1R (Ab-1161) Antibody (left) or the same antibody preincubated with blocking peptide (right).

200; 95

insulin like growth factor 1 receptor

insulin-like growth factor 1 receptor

Insulin-like growth factor 1 receptor

IGF-I receptor  [Similar Products]

This receptor binds insulin-like growth factor with a high affinity. It has tyrosine kinase activity. The insulin-like growth factor I receptor plays a critical role in transformation events. Cleavage of the precursor generates alpha and beta subunits. It is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, May 2014]

IGF1R: a receptor tyrosine kinase that binds insulin-like growth factor 1 (IGF1) with a high affinity and IGF2 with a lower affinity. Functions as an anti-apoptotic agent by enhancing cell survival. Can play a critical role in transformation events. Cleavage of the precursor generates alpha and beta subunits. Tetramer of 2 alpha and 2 beta chains linked by disulfide bonds. The alpha chains contribute to the formation of the ligand- binding domain, while the beta chain carries the kinase domain. Interacts with PIK3R1 and with the PTB/PID domains of IRS1 and SHC1 in vitro when autophosphorylated on tyrosine residues. Mutated in rare cases of pre- and post-natal growth retardation. One SNP associated with increased human longevity. Increased expression of IGF1R and other pathway members associated with progression and malignancy in a range of cancers. Inhibitors: AG1024, AEW541.

Protein type: EC 2.7.10.1; InsR family; Kinase, protein; Membrane protein, integral; Protein kinase, TK; Protein kinase, tyrosine (receptor); TK group

Chromosomal Location of Human Ortholog: 15q26.3

Cellular Component: integral to plasma membrane; intracellular membrane-bound organelle; membrane; plasma membrane; receptor complex

Molecular Function: identical protein binding; insulin binding; insulin receptor binding; insulin receptor substrate binding; insulin-like growth factor binding; insulin-like growth factor I binding; insulin-like growth factor receptor activity; phosphoinositide 3-kinase binding; protein binding; protein-tyrosine kinase activity

Biological Process: immune response; inactivation of MAPKK activity; insulin receptor signaling pathway; insulin-like growth factor receptor signaling pathway; negative regulation of apoptosis; phosphoinositide 3-kinase cascade; phosphoinositide-mediated signaling; positive regulation of cell migration; positive regulation of cell proliferation; positive regulation of DNA replication; protein amino acid autophosphorylation; protein tetramerization; regulation of JNK cascade; signal transduction

Disease: Insulin-like Growth Factor I, Resistance To

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