Glycine Dehydrogenase, Polyclonal Antibody

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

IgG

Rabbit

The antibody is a rabbit polyclonal antibody raised against GLDC. It has beenselected for its ability to recognize GLDC in immunohistochemical staining andwestern blotting.

Affinity Chromatography

Liquid

200ug/ml (lot specific)

Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.

Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.

Small volumes of anti-GLDC antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)

Western blotting: 0.2-2ug/mL;1:250-2500
Immunohistochemistry: 5-20ug/mL;1:25-100
Immunocytochemistry: 5-20ug/mL;1:25-100
Optimal working dilutions must be determined by end user.

Western Blot: Sample: Recombinant protein.

DAB staining on fromalin fixed paraffin-embedded liver tissue)

113,267 Da

glycine decarboxylase

glycine dehydrogenase (decarboxylating), mitochondrial

Glycine dehydrogenase (decarboxylating), mitochondrial

GLDC: The glycine cleavage system catalyzes the degradation of glycine. The P protein binds the alpha-amino group of glycine through its pyridoxal phosphate cofactor; CO(2) is released and the remaining methylamine moiety is then transferred to the lipoamide cofactor of the H protein. Defects in GLDC are a cause of non-ketotic hyperglycinemia (NKH); also known as glycine encephalopathy (GCE). NKH is an autosomal recessive disease characterized by accumulation of a large amount of glycine in body fluid and by severe neurological symptoms. Belongs to the GcvP family.

Protein type: Amino Acid Metabolism - glycine, serine and threonine; EC 1.4.4.2; Oxidoreductase

Chromosomal Location of Human Ortholog: 19 C1|19 24.87 cM

Cellular Component: glycine cleavage complex; mitochondrion; nucleus; plasma membrane

Molecular Function: catalytic activity; enzyme binding; glycine binding; glycine dehydrogenase (decarboxylating) activity; lyase activity; oxidoreductase activity; protein dimerization activity; protein homodimerization activity; pyridoxal phosphate binding

Biological Process: amino acid metabolic process; glycine catabolic process; glycine decarboxylation via glycine cleavage system; glycine metabolic process

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