Product Name
ACTC1, siRNA
Full Product Name
ACTC1 siRNA (Human)
Product Synonym Names
ACTC; Actin alpha cardiac muscle 1; Alpha-cardiac actin
Product Gene Name
ACTC1 sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P68032
Specificity
ACTC1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ACTC1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of ACTC1 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
ACTC1 sirna
siRNA to inhibit ACTC1 expression using RNA interference
Applications Tested/Suitable for ACTC1 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for ACTC1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_005150.1
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NCBI GenBank Nucleotide #
NM_005159.4
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UniProt Primary Accession #
P68032
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UniProt Secondary Accession #
P04270[Other Products]
UniProt Related Accession #
P68032[Other Products]
Molecular Weight
42,019 Da
NCBI Official Full Name
actin, alpha cardiac muscle 1 proprotein
NCBI Official Synonym Full Names
actin, alpha, cardiac muscle 1
NCBI Official Symbol
ACTC1 [Similar Products]
NCBI Official Synonym Symbols
ACTC; ASD5; CMD1R; CMH11; LVNC4
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NCBI Protein Information
actin, alpha cardiac muscle 1
UniProt Protein Name
Actin, alpha cardiac muscle 1
UniProt Synonym Protein Names
Alpha-cardiac actin
UniProt Gene Name
ACTC1 [Similar Products]
UniProt Synonym Gene Names
ACTC [Similar Products]
UniProt Entry Name
ACTC_HUMAN
NCBI Summary for ACTC1
Actins are highly conserved proteins that are involved in various types of cell motility. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to four others. The protein encoded by this gene belongs to the actin family which is comprised of three main groups of actin isoforms, alpha, beta, and gamma. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. Defects in this gene have been associated with idiopathic dilated cardiomyopathy (IDC) and familial hypertrophic cardiomyopathy (FHC). [provided by RefSeq, Jul 2008]
UniProt Comments for ACTC1
ACTC1: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Belongs to the actin family.
Protein type: Cell development/differentiation; Cytoskeletal; Myosin-binding; Contractile; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 15q14
Cellular Component: I band; extracellular space; sarcomere; focal adhesion; membrane; cytoplasm; actin filament; cytosol
Molecular Function: myosin binding; ATPase activity; ATP binding
Biological Process: response to drug; actin filament-based movement; muscle thin filament assembly; apoptosis; metabolic process; actomyosin structure organization and biogenesis; cardiac myofibril assembly; muscle filament sliding; response to ethanol; heart contraction; cardiac muscle morphogensis; actin-myosin filament sliding; negative regulation of apoptosis; cardiac muscle contraction
Disease: Atrial Septal Defect 5; Cardiomyopathy, Familial Hypertrophic, 11; Cardiomyopathy, Dilated, 1r
Research Articles on ACTC1
1. In this study, there was no association of the analyzed SNPs located in RASGRF1. GJD2, and ACTC1 with pathological myopia.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
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