PNP, Polyclonal Antibody

Purine nucleoside phosphorylase; PNP; Inosine phosphorylase; Inosine-guanosine phosphorylase; PNP; NP

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit Ig

Rabbit

This PNP antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 145-178 amino acids from the Central region of human PNP.

Purified Rabbit Polyclonal Antibody (Pab)

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Vial Concentration: 0.5 (lot specific)

Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Small volumes of anti-PNP antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

The purine nucleoside phosphorylases catalyze the phosphorolytic breakdown of the N-glycosidic bond in the beta- (deoxy)ribonucleoside molecules, with the formation of the corresponding free purine bases and pentose-1-phosphate.

Crown Antibodies; Neuroscience

Western Blot (WB), ELISA (EIA)

WB~~1:1000

Western blot analysis of lysates from Daudi, HT-1080, K562, MCF-7, MDA-MB-231 cell line (from left to right), using PNP Antibody (Center). MBS9212480 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.

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purine nucleoside phosphorylase

purine nucleoside phosphorylase

Purine nucleoside phosphorylase

NP; PNP  [Similar Products]

PNPH_HUMAN

This gene encodes an enzyme which reversibly catalyzes the phosphorolysis of purine nucleosides. The enzyme is trimeric, containing three identical subunits. Mutations which result in nucleoside phosphorylase deficiency result in defective T-cell (cell-mediated) immunity but can also affect B-cell immunity and antibody responses. Neurologic disorders may also be apparent in patients with immune defects. A known polymorphism at aa position 51 that does not affect enzyme activity has been described. A pseudogene has been identified on chromosome 2. [provided by RefSeq, Jul 2008]

NP: a metabolic enzyme with purine-nucleoside phosphorylase activity. Defects are the cause of nucleoside phosphorylase deficiency (NP deficiency), with severe T-cell immunodeficiency with neurologic disorder in children.

Protein type: Transferase; EC 2.4.2.1; Nucleotide Metabolism - pyrimidine; Nucleotide Metabolism - purine; Cofactor and Vitamin Metabolism - nicotinate and nicotinamide

Chromosomal Location of Human Ortholog: 14q13.1

Cellular Component: cytoskeleton; cytoplasm; intracellular; cytosol

Molecular Function: purine binding; nucleoside binding; drug binding; phosphate binding; purine-nucleoside phosphorylase activity

Biological Process: response to drug; nucleobase, nucleoside and nucleotide metabolic process; nicotinamide riboside catabolic process; inosine catabolic process; nucleobase, nucleoside, nucleotide and nucleic acid metabolic process; purine nucleotide catabolic process; positive regulation of T cell proliferation; immune response; positive regulation of alpha-beta T cell differentiation; purine salvage; purine base metabolic process

Disease: Purine Nucleoside Phosphorylase Deficiency

Williams S.R.,et al.Nucleic Acids Res. 12:5779-5787(1984).
Williams S.R.,et al.J. Biol. Chem. 262:2332-2338(1987).
Yu L.,et al.Environ. Health Perspect. 111:1421-1427(2003).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Ebert L.,et al.Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases.

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