Product Name
CACNG2, siRNA
Full Product Name
CACNG2 siRNA (Mouse)
Product Synonym Names
STG; Voltage-dependent calcium channel gamma-2 subunit; Neuronal voltage-gated calcium channel gamma-2 subunit; Stargazin; Transmembrane AMPAR regulatory protein gamma-2; TARP gamma-2
Product Gene Name
CACNG2 sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for O88602
Specificity
CACNG2 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse CACNG2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of CACNG2 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
CACNG2 sirna
siRNA to inhibit CACNG2 expression using RNA interference
Applications Tested/Suitable for CACNG2 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for CACNG2. It may not necessarily be applicable to this product.
NCBI Accession #
NP_031609.1
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NCBI GenBank Nucleotide #
NM_007583.2
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UniProt Primary Accession #
O88602
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UniProt Related Accession #
O88602[Other Products]
Molecular Weight
35,895 Da
NCBI Official Full Name
voltage-dependent calcium channel gamma-2 subunit
NCBI Official Synonym Full Names
calcium channel, voltage-dependent, gamma subunit 2
NCBI Official Symbol
Cacng2 [Similar Products]
NCBI Official Synonym Symbols
stg; wag; waggler; AW060990; stargazer; stargazin; B230105C07Rik; B930041E13Rik
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NCBI Protein Information
voltage-dependent calcium channel gamma-2 subunit
UniProt Protein Name
Voltage-dependent calcium channel gamma-2 subunit
UniProt Synonym Protein Names
Neuronal voltage-gated calcium channel gamma-2 subunit; Stargazin; Transmembrane AMPAR regulatory protein gamma-2; TARP gamma-2
Protein Family
Voltage-dependent calcium channel
UniProt Gene Name
Cacng2 [Similar Products]
UniProt Synonym Gene Names
Stg; TARP gamma-2 [Similar Products]
UniProt Entry Name
CCG2_MOUSE
UniProt Comments for CACNG2
Stargazin: Regulates the trafficking and gating properties of AMPA- selective glutamate receptors (AMPARs). Promotes their targeting to the cell membrane and synapses and modulates their gating properties by slowing their rates of activation, deactivation and desensitization. Does not show subunit-specific AMPA receptor regulation and regulates all AMPAR subunits. Thought to stabilize the calcium channel in an inactivated (closed) state. The L-type calcium channel is composed of five subunits: alpha-1, alpha-2/delta, beta and gamma. Interacts with the PDZ domains of DLG4/PSD-95 and DLG1/SAP97. May interact with GOPC. Acts as an auxiliary subunit for AMPA-selective glutamate receptors (AMPARs). Found in a complex with GRIA1, GRIA2, GRIA3, GRIA4, CNIH2, CNIH3, CACNG3, CACNG4, CACNG5, CACNG7 and CACNG8. Interacts with GRIA1. Brain. Belongs to the PMP-22/EMP/MP20 family. CACNG subfamily.
Protein type: Membrane protein, integral; Membrane protein, multi-pass; Channel, calcium
Cellular Component: membrane; integral to membrane; voltage-gated calcium channel complex
Molecular Function: voltage-gated calcium channel activity; ionotropic glutamate receptor binding; calcium channel activity; voltage-gated ion channel activity
Biological Process: membrane depolarization; regulation of membrane potential; transport; calcium ion transport; membrane hyperpolarization; transmission of nerve impulse; ion transport; neuromuscular junction development; neurological system process
Research Articles on CACNG2
1. in the absence of stargazin, the refinement of the retinogeniculate synapse is specifically disrupted during the experience-dependent phase. Stargazin expression and phosphorylation increased with visual deprivation.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
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