Full Product Name
PNP Antibody
Product Synonym Names
PNP; Inosine phosphorylase; Inosine-guanosine phosphorylase; PNP; NP
Product Gene Name
anti-PNP antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P00491
Purity/Purification
Antigen Affinity Purified
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Immunogen
Recombinant human Purine nucleoside phosphorylase protein (1-289aa)
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-47333 / sc-47335 / sc-47334 / sc-47336
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Other Notes
Small volumes of anti-PNP antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-PNP antibody
The purine nucleoside phosphorylases catalyze the phosphorolytic breakdown of the N-glycosidic bond in the beta-(deoxy)ribonucleoside molecules, with the formation of the corresponding free purine bases and pentose-1-phosphate.
Applications Tested/Suitable for anti-PNP antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-PNP antibody
Recommended dilution: WB:1:500-2000
IHC:1:20-1:200
Western Blot (WB) of anti-PNP antibody
Western blot
All lanes: PNP antibody at 10ug/ml
Lane 1:Jurkat whole cell lysate
Lane 2:k562 whole cell lysate
Secondary
Goat polyclonal to rabbit at 1/10000 dilution
Predicted band size: 32kDa
Observed band size: 32kDa
Immunohistochemistry (IHC) of anti-PNP antibody
Immunohistochemistry of paraffin-embedded human brain tissue using MBS1491860 at dilution 1:100
NCBI/Uniprot data below describe general gene information for PNP. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000261.2
[Other Products]
NCBI GenBank Nucleotide #
NM_000270.3
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UniProt Primary Accession #
P00491
[Other Products]
UniProt Secondary Accession #
Q15160; Q5PZ03; B2R8S5; D3DS00[Other Products]
UniProt Related Accession #
P00491[Other Products]
Molecular Weight
32,118 Da
NCBI Official Full Name
purine nucleoside phosphorylase
NCBI Official Synonym Full Names
purine nucleoside phosphorylase
NCBI Official Symbol
PNP [Similar Products]
NCBI Official Synonym Symbols
NP; PUNP; PRO1837
[Similar Products]
NCBI Protein Information
purine nucleoside phosphorylase
UniProt Protein Name
Purine nucleoside phosphorylase
UniProt Synonym Protein Names
Inosine phosphorylase; Inosine-guanosine phosphorylase
Protein Family
Polyribonucleotide nucleotidyltransferase
UniProt Gene Name
PNP [Similar Products]
UniProt Synonym Gene Names
NP; PNP [Similar Products]
UniProt Entry Name
PNPH_HUMAN
NCBI Summary for PNP
This gene encodes an enzyme which reversibly catalyzes the phosphorolysis of purine nucleosides. The enzyme is trimeric, containing three identical subunits. Mutations which result in nucleoside phosphorylase deficiency result in defective T-cell (cell-mediated) immunity but can also affect B-cell immunity and antibody responses. Neurologic disorders may also be apparent in patients with immune defects. A known polymorphism at aa position 51 that does not affect enzyme activity has been described. A pseudogene has been identified on chromosome 2. [provided by RefSeq, Jul 2008]
UniProt Comments for PNP
NP: a metabolic enzyme with purine-nucleoside phosphorylase activity. Defects are the cause of nucleoside phosphorylase deficiency (NP deficiency), with severe T-cell immunodeficiency with neurologic disorder in children.
Protein type: EC 2.4.2.1; Cofactor and Vitamin Metabolism - nicotinate and nicotinamide; Nucleotide Metabolism - purine; Transferase; Nucleotide Metabolism - pyrimidine
Chromosomal Location of Human Ortholog: 14q13.1
Cellular Component: cytoplasm; cytoskeleton; cytosol; intracellular; nucleus
Molecular Function: drug binding; nucleoside binding; phosphate binding; purine binding; purine-nucleoside phosphorylase activity
Biological Process: immune response; inosine catabolic process; nicotinamide riboside catabolic process; nucleobase, nucleoside and nucleotide metabolic process; nucleobase, nucleoside, nucleotide and nucleic acid metabolic process; positive regulation of alpha-beta T cell differentiation; positive regulation of T cell proliferation; purine base metabolic process; purine nucleotide catabolic process; purine salvage; response to drug
Disease: Purine Nucleoside Phosphorylase Deficiency
Research Articles on PNP
1. Data show that [15N, 2H]His8-purine nucleoside phosphorylase (PNP) had reduced catalytic site chemistry larger than proportional to the enzymatic mass difference.
Precautions
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