Purine Nucleoside Phosphorylase, ELISA Kit

For Research Use Only. Not for use in diagnostic procedures.

Chromosome: 14; NC_000014.8 (20937538..20946165). Location: 14q13.1

Store all reagents at 2-8 degree C

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Small volumes of PNP elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS034356 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Purine Nucleoside Phosphorylase (PNP) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PNP. The ELISA analytical biochemical technique of the MBS034356 kit is based on PNP antibody-PNP antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PNP antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PNP. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

32,118 Da

purine nucleoside phosphorylase

purine nucleoside phosphorylase; inosine phosphorylase; inosine-guanosine phosphorylase; purine-nucleoside:orthophosphate ribosyltransferase

Purine nucleoside phosphorylase

NP; PNP  [Similar Products]

PNPH_HUMAN

This gene encodes an enzyme which reversibly catalyzes the phosphorolysis of purine nucleosides. The enzyme is trimeric, containing three identical subunits. Mutations which result in nucleoside phosphorylase deficiency result in defective T-cell (cell-mediated) immunity but can also affect B-cell immunity and antibody responses. Neurologic disorders may also be apparent in patients with immune defects. A known polymorphism at aa position 51 that does not affect enzyme activity has been described. A pseudogene has been identified on chromosome 2. [provided by RefSeq, Jul 2008]

NP: a metabolic enzyme with purine-nucleoside phosphorylase activity. Defects are the cause of nucleoside phosphorylase deficiency (NP deficiency), with severe T-cell immunodeficiency with neurologic disorder in children.

Protein type: Cofactor and Vitamin Metabolism - nicotinate and nicotinamide; Transferase; EC 2.4.2.1; Nucleotide Metabolism - purine; Nucleotide Metabolism - pyrimidine

Chromosomal Location of Human Ortholog: 14q13.1

Cellular Component: cytoskeleton; cytoplasm; intracellular; cytosol

Molecular Function: purine binding; nucleoside binding; drug binding; phosphate binding; purine-nucleoside phosphorylase activity

Biological Process: response to drug; nicotinamide riboside catabolic process; inosine catabolic process; nucleobase, nucleoside and nucleotide metabolic process; nucleobase, nucleoside, nucleotide and nucleic acid metabolic process; purine nucleotide catabolic process; immune response; positive regulation of T cell proliferation; purine base metabolic process; positive regulation of alpha-beta T cell differentiation; purine salvage

Disease: Purine Nucleoside Phosphorylase Deficiency

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