Full Product Name
PFKM (Phospho-Ser74) Antibody
Product Synonym Names
6 Phosphofructokinase Muscle Type; 6-phosphofructokinase; 6-phosphofructokinase muscle type; EC 2.7.1.1; EC 2.7.1.11; GSD7; K6PF_HUMAN; MGC8699; muscle type; PFK, muscle type; PFK-A; PFKA; PFKL; PFKM; PFKP; PFKX; Phosphofructo 1 Kinase Isozyme A; Phosphofructo-1-kinase isozyme A; Phosphofructokinase 1; Phosphofructokinase M; Phosphofructokinase, muscle; Phosphofructokinase, muscle type; Phosphofructokinase, polypeptide X; Phosphofructokinase-M; Phosphohexokinase;
Product Gene Name
anti-PFKM antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P08237
Species Reactivity
Human, Mouse, Rat
Specificity
Phospho-PFKM (Phospho-Ser74) Antibody detects endogenous levels of PFKM only when phosphorylated at Ser74
Purity/Purification
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Form/Format
In phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1.0mg/mL (lot specific)
Immunogen
A synthesized peptide derived from human PFKM around the phosphorylation site of Ser74
Preparation and Storage
Store at -20 degree C.
Other Notes
Small volumes of anti-PFKM antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Product Categories/Family for anti-PFKM antibody
Phospho-specific Ab
Applications Tested/Suitable for anti-PFKM antibody
Western Blot (WB)
Application Notes for anti-PFKM antibody
WB: 1:500-1:2000
Western Blot (WB) of anti-PFKM antibody
Western blot analysis of Phospho-PFKM (Ser74) in lysates of HeLa?, using Phospho-PFKM (Ser74) Antibody(12927).
NCBI/Uniprot data below describe general gene information for PFKM. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000280.1
[Other Products]
NCBI GenBank Nucleotide #
NM_000289.5
[Other Products]
UniProt Primary Accession #
P08237
[Other Products]
UniProt Secondary Accession #
Q16814; Q16815; Q6ZTT1; J3KNX3[Other Products]
UniProt Related Accession #
P08237[Other Products]
NCBI Official Full Name
ATP-dependent 6-phosphofructokinase, muscle type isoform 2
NCBI Official Synonym Full Names
phosphofructokinase, muscle
NCBI Official Symbol
PFKM [Similar Products]
NCBI Official Synonym Symbols
GSD7; PFK1; PFKA; PFKX; PFK-1; PFK-A; ATP-PFK; PPP1R122
[Similar Products]
NCBI Protein Information
ATP-dependent 6-phosphofructokinase, muscle type
UniProt Protein Name
ATP-dependent 6-phosphofructokinase, muscle type
UniProt Synonym Protein Names
6-phosphofructokinase type A; Phosphofructo-1-kinase isozyme A; PFK-A; Phosphohexokinase
Protein Family
ATP-dependent 6-phosphofructokinase
UniProt Gene Name
PFKM [Similar Products]
UniProt Synonym Gene Names
PFKX; ATP-PFK; PFK-M [Similar Products]
NCBI Summary for PFKM
Three phosphofructokinase isozymes exist in humans: muscle, liver and platelet. These isozymes function as subunits of the mammalian tetramer phosphofructokinase, which catalyzes the phosphorylation of fructose-6-phosphate to fructose-1,6-bisphosphate. Tetramer composition varies depending on tissue type. This gene encodes the muscle-type isozyme. Mutations in this gene have been associated with glycogen storage disease type VII, also known as Tarui disease. Alternatively spliced transcript variants have been described.[provided by RefSeq, Nov 2009]
UniProt Comments for PFKM
Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.
Research Articles on PFKM
1. Data indicate that 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase3 (PFKFB3) and phosphofructokinase (PFK1) expression allows discrimination between induced pluripotent stem cells (iPS) and cancer stem cells (CSCs).
Precautions
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Disclaimer
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