Follitropin subunit beta (FSHB), ELISA Kit

For Research Use Only. Not for use in diagnostic procedures.

This assay has high sensitivity and excellent specificity for detection of FSHB. No significant cross-reactivity or interference between FSHB and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between FSHB and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of FSHB elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS7234844 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Follitropin subunit beta (FSHB) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing FSHB. The ELISA analytical biochemical technique of the MBS7234844 kit is based on FSHB antibody-FSHB antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect FSHB antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, FSHB. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Principle of the assay: FSHB ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-FSHB antibody and an FSHB-HRP conjugate. The assay sample and buffer are incubated together with FSHB-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FSHB concentration since FSHB from samples and FSHB-HRP conjugate compete for the anti-FSHB antibody . Since the number of sites is limited, as more sites are occupied by FSHB from the sample, fewer sites are left to bind FSHB-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FSHB concentration in each sample is interpolated from this standard curve.

14,700 Da

follicle stimulating hormone, beta polypeptide

follitropin subunit beta; FSH-B; FSH-beta; follitropin beta chain; follitropin, beta chain; follicle-stimulating hormone beta subunit

Follitropin subunit beta

FSH-B; FSH-beta  [Similar Products]

FSHB_HUMAN

The pituitary glycoprotein hormone family includes follicle-stimulating hormone, luteinizing hormone, chorionic gonadotropin, and thyroid-stimulating hormone. All of these glycoproteins consist of an identical alpha subunit and a hormone-specific beta subunit. This gene encodes the beta subunit of follicle-stimulating hormone. In conjunction with luteinizing hormone, follicle-stimulating hormone induces egg and sperm production. Alternative splicing results in two transcript variants encoding the same protein. [provided by RefSeq, Jul 2008]

FSHB: Stimulates development of follicle and spermatogenesis in the reproductive organs. Defects in FSHB are a cause of isolated follicle- stimulating hormone deficiency (IFSHD). Selective follicle-stimulating hormone deficiency is an uncommon cause of infertility, producing amenorrhea and hypogonadism in women and oligo or azoospermia with normal testosterone levels in normally virilised men. Belongs to the glycoprotein hormones subunit beta family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 11p13

Cellular Component: cytoplasm; extracellular region

Molecular Function: follicle-stimulating hormone activity; protein binding; hormone activity

Biological Process: progesterone biosynthetic process; follicle-stimulating hormone signaling pathway; peptide hormone processing; female gamete generation; Sertoli cell proliferation; female pregnancy; signal transduction; regulation of osteoclast differentiation; cellular protein metabolic process; transforming growth factor beta receptor signaling pathway; positive regulation of cell proliferation; positive regulation of bone resorption; positive regulation of transcription from RNA polymerase II promoter; positive regulation of cell migration

Disease: Follicle-stimulating Hormone Deficiency, Isolated

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