Biotinidase (BTD), ELISA Kit

For Research Use Only. Not for use in diagnostic procedures.

Store at 2 to 8 degree C for 6 months.

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of BTD elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS167321 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Biotinidase (BTD) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing BTD. The ELISA analytical biochemical technique of the MBS167321 kit is based on BTD antibody-BTD antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect BTD antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, BTD. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Intended Uses: This sandwich kit is for the accurate quantitative detection of human Biotinidase (also known as BTD) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human BTD antibody. BTD present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human BTD Antibody is added and binds to BTD in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated BTD antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human BTD. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

61,510 Da

biotinidase

biotinidase

Biotinidase

Biotinase  [Similar Products]

The protein encoded by this gene functions to recycle protein-bound biotin by cleaving biocytin (biotin-epsilon-lysine), a normal product of carboxylase degradation, resulting in regeneration of free biotin. The encoded protein has also been shown to have biotinyl transferase activity. Mutations in this gene are associated with biotinidase deficiency. Multiple transcript variants encoding different isoforms have been described. [provided by RefSeq, Aug 2013]

Catalytic release of biotin from biocytin, the product of biotin-dependent carboxylases degradation.CautionIt is uncertain whether Met-1 or Met-21 is the initiator.

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