Product Name
Growth Hormone (rtGH), Recombinant Protein
Full Product Name
Recombinant Rainbow Trout Growth Hormone
Product Synonym Names
GH1; GH; GHN; GH-N; hGH-N; Pituitary growth hormone; Growth hormone 1; Somatotropin; GH Rainbow Trout; Growth Hormone Rainbow Trout (Oncorhynchus mykiss) Recombinant
Product Gene Name
rtGH recombinant protein
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence
AIENQRLFNI AVSRVQHLHL LAQKMFNDFD GTLLPDERRQ LNKIFLLDFC NSDSIVSPVD KHETQKSSVL KLLHISFRLI ESWEYPSQTL IISNSLMVRN ANQISEKLSD LKVGINLLIT GSQDGVLSLD DNDSQQLPPY GNYYQNLGGD GNVRRNYELL ACFKKDMHKV ETYLTVAKCR KSLEANCTL
3D Structure
ModBase 3D Structure for P09538
Purity/Purification
Greater than 95.0% as determined by:(a) Analysis by SEC-HPLC.(b) Analysis by SDS-PAGE
Form/Format
Sterile filtered white lyophilized (freeze-dried) powder
Protein was lyophilized from a concentrated (1mg/ml) solution with 0.5% NaHCO3. Adjusted to pH-8
Biological Activity
Somatotropin Rainbow Trout (Oncorhynchus mykiss) Recombinant is
biologically active in PDF-P1 3B9 cells stable transfected with rabbit GH receptors, though its activity is about 10 fold lower than that of human GH
Solubility
It is recommended to reconstitute the lyophilized Growth-Hormone Rainbow Trout (Oncorhynchus mykiss) in 0.4% NaHCO3 or water adjusted to pH 8-9, not less than 100ug/ml and not more than 3mg/ml, which can then be further diluted to other aqueous solutions, preferably in a presence of a carrier protein such as BSA or similar
Preparation and Storage
Although stable at room temperature for at least two weeks, should be stored desiccated below-18 degree C.
Upon reconstitution and filter sterilization GH can be stored at 4 degree C, pH 9 for up to 4 weeks.
For long term storage and more diluted solutions it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles
Other Notes
Small volumes of rtGH recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
rtGH recombinant protein
Description: Somatotropin Rainbow Trout (Oncorhynchus mykiss) Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain containing 188 amino acids with an additional Ala at the N-terminus and having a molecular mass of 21, 535 Dalton. The Rainbow Trout (Oncorhynchus mykiss) Growth-Hormone Recombinant is purified by proprietary chromatographic techniques.
Introduction: GH is a member of the somatotropin/prolactin family of hormones which play an important role in growth control. The gene, along with four other related genes, is located at the growth hormone locus on chromosome 17 where they are interspersed in the same transcriptional orientation; an arrangement which is thought to have evolved by a series of gene duplications. The five genes share a remarkably high degree of sequence identity. Alternative splicing generates additional isoforms of each of the five growth hormones, leading to further diversity and potential for specialization. This particular family member is expressed in the pituitary but not in placental tissue as is the case for the other four genes in the growth hormone locus. Mutations in or deletions of the gene lead to growth hormone deficiency and short stature.
Product Categories/Family for rtGH recombinant protein
CYTOKINES AND GROWTH FACTORS; Growth Factors; Growth Hormone; GH Rainbow Trout
NCBI/Uniprot data below describe general gene information for rtGH. It may not necessarily be applicable to this product.
NCBI Accession #
P09538.2
[Other Products]
UniProt Primary Accession #
P09538
[Other Products]
UniProt Related Accession #
P09538[Other Products]
Molecular Weight
23,795 Da
NCBI Official Full Name
Somatotropin-1
NCBI Official Symbol
gh1 [Similar Products]
NCBI Protein Information
somatotropin-1
UniProt Protein Name
Somatotropin-1
UniProt Synonym Protein Names
Growth hormone 1
Protein Family
Growth hormone
UniProt Gene Name
gh1 [Similar Products]
UniProt Comments for rtGH
Growth hormone plays an important role in growth control and is involved in the regulation of several anabolic processes. Implicated as an osmoregulatory substance important for seawater adaptation.
Research Articles on rtGH
1. Findings show similar regulatory growth hormone (GH) and insulin-like growth factor 1 (IGF-1) responses in both Atlantic salmon and rainbow trout.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
