ATP6V1B1, Antibody

Vacuolar ATP synthase subunit B; kidney isoform; V-ATPase subunit B 1; Vacuolar proton pump subunit B 1; Endomembrane proton pump 58 kDa subunit; ATP6V1B1; ATP6B1; VATB; VPP3

For Research Use Only. Not for use in diagnostic procedures.

ATP6V1B1 Antibody detects endogenous levels of total ATP6V1B1

Immunogen affinity purified

In phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Store at-20 degree C for 1 year

Small volumes of anti-ATP6V1B1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Western Blot (WB), Immunohistochemistry (IHC)

WB: 1:500~1:1000
IHC: 1:50~1:200

Western blot analysis of extracts from HepG2 cells, using ATP6V1B1 antibody.

60 KD

ATPase H+ transporting V1 subunit B1

V-type proton ATPase subunit B, kidney isoform

V-type proton ATPase subunit B, kidney isoform

ATP6B1; VATB; VPP3; V-ATPase subunit B 1  [Similar Products]

This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c'', and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is one of two V1 domain B subunit isoforms and is found in the kidney. Mutations in this gene cause distal renal tubular acidosis associated with sensorineural deafness. [provided by RefSeq, Jul 2008]

Non-catalytic subunit of the peripheral V1 complex of vacuolar ATPase. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells.

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