26S Proteasome Non-ATPase Regulatory Subunit 3 (PSMD3), ELISA Kit

26S Proteasome Regulatory Subunit RPN3; 26S Proteasome Regulatory Subunit S3; Proteasome Subunit p58

For Research Use Only. Not for use in diagnostic procedures.

This assay has high sensitivity and excellent specificity for detection of PSMD3. No significant cross-reactivity or interference between PSMD3 and analogues was observed.

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of PSMD3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS456915 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the 26S Proteasome Non-ATPase Regulatory Subunit 3 (PSMD3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PSMD3. The ELISA analytical biochemical technique of the MBS456915 kit is based on PSMD3 antibody-PSMD3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PSMD3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PSMD3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of PSMD3 in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to PSMD3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to PSMD3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PSMD3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of PSMD3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

41,184 Da

proteasome 26S subunit, non-ATPase 3

26S proteasome non-ATPase regulatory subunit 3

26S proteasome non-ATPase regulatory subunit 3

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. This gene encodes a member of the proteasome subunit S3 family that functions as one of the non-ATPase subunits of the 19S regulator lid. Single nucleotide polymorphisms in this gene are associated with neutrophil count. [provided by RefSeq, Jul 2012]

PSMD3: Acts as a regulatory subunit of the 26 proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins. Belongs to the proteasome subunit S3 family.

Protein type: Protease; Proteasome complex

Chromosomal Location of Human Ortholog: 17q21.1

Cellular Component: cytosol; extracellular exosome; extracellular region; ficolin-1-rich granule lumen; membrane; nucleoplasm; nucleus; proteasome complex; proteasome regulatory particle, lid subcomplex; secretory granule lumen

Molecular Function: enzyme regulator activity; protein binding

Biological Process: anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; Fc-epsilon receptor signaling pathway; MAPK cascade; negative regulation of canonical Wnt signaling pathway; negative regulation of G2/M transition of mitotic cell cycle; negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle; neutrophil degranulation; NIK/NF-kappaB signaling; positive regulation of canonical Wnt signaling pathway; positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; post-translational protein modification; proteasomal ubiquitin-dependent protein catabolic process; protein deubiquitination; protein polyubiquitination; regulation of cellular amino acid metabolic process; regulation of hematopoietic stem cell differentiation; regulation of mRNA stability; SCF-dependent proteasomal ubiquitin-dependent protein catabolic process; stimulatory C-type lectin receptor signaling pathway; T cell receptor signaling pathway; transmembrane transport; tumor necrosis factor-mediated signaling pathway; ubiquitin-dependent protein catabolic process; Wnt receptor signaling pathway, planar cell polarity pathway

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