Cytokeratin 14, Polyclonal Antibody

CK-14; cytokeratin 14; Cytokeratin-14; EBS3; EBS4; K14; K1C14; keratin 14; Keratin; type I cytoskeletal 14; Keratin-14; KRT14; NFJ

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

IgG

Rabbit

Cytokeratin 14 Antibody detects endogenous levels of Cytokeratin 14

Affinity Chromatography

Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Store at 4 degree C for short term.
Store at-20 degree C for long term.
Avoid freeze/thaw cycle.

Small volumes of anti-CK14 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

K14 a type I cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. There are two types of cytoskeletal and microfibrillar keratin: type I (acidic; 40-55 kDa) [K9 to K20] and type II (neutral to basic; 56-70 kDa) [K1 to K8]. Both a basic and an acidic keratin are required for filament assembly.

Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)

WB: 1:1000-1:2000
IHC: 1:50-1:200
ICC/IF: 1:50-1:200

Western blot analysis of Cytokeratin 14 expression in A431 cell lysate.

Immunohistochemical analysis of paraffin-embedded human stomach, using Cytokeratin 14 Antibody.

Immunofluorescent analysis of HACAT cells, using Cytokeratin 14 Antibody.

53kDa

keratin 14

keratin, type I cytoskeletal 14

Keratin, type I cytoskeletal 14

CK-14; K14  [Similar Products]

This gene encodes a member of the keratin family, the most diverse group of intermediate filaments. This gene product, a type I keratin, is usually found as a heterotetramer with two keratin 5 molecules, a type II keratin. Together they form the cytoskeleton of epithelial cells. Mutations in the genes for these keratins are associated with epidermolysis bullosa simplex. At least one pseudogene has been identified at 17p12-p11. [provided by RefSeq, Jul 2008]

The nonhelical tail domain is involved in promoting KRT5-KRT14 filaments to self-organize into large bundles and enhances the mechanical properties involved in resilience of keratin intermediate filaments in vitro.

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