Product Name
Cytokeratin 14 (KRT14), Monoclonal Antibody
Full Product Name
Cytokeratin 14 Antibody
Product Gene Name
anti-KRT14 antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P02533
Buffer
Tris Buffer, ph7.4, 1% BSA, <0.1% NaN3
IHC Positive Control
Squamous Cell Carcinoma
IHC Suggested Protocol
1. Tissues are fixed with 10% neutral buffered formalin, cut to ~4um, and placed on a positively charged glass slide.
2. The prepared slide is baked for at least 30 minutes and at most 24 hours at 55-65 degree C.
3. Pretreatment - Perform heat-induced epitope retrieval (HIER) at pH 6 for 10 to 30 minutes.
4. Block Block with peroxidase solution for 5 minutes at RT.
5. Primary Ab Dilute antibodies 1:100 1:200 and incubate for 10 - 30 minutes at RT.
6. Secondary Ab - Incubate for 20 - 30 minutes at RT.
7. Substrate - Incubate DAB or Fast Red for 5 - 10 minutes at RT.
8. Counterstain - Counterstain with hematoxylin for 30 seconds to 5 minutes, rinse with distilled water and blueing solution for 30 seconds.
9. Dehydrate and apply coverslip.
(Each researcher should optimize the protocol for their experimental model system.)
Preparation and Storage
Store at 2-8 degree C. Do not freeze.
Other Notes
Small volumes of anti-KRT14 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-KRT14 antibody
CK14 (Cytokeratin 14) is part of the type I keratin family proteins, and can be found in squamous epithelial basal cells, myoepithelium, some glandular epithelia, and mesothelial cells. Anti-Cytokeratin 14 antibodies can be useful in identifying squamous cell carcinomas from other epithelial tumors, and for classifying metaplastic breast carcinomas.
Applications Tested/Suitable for anti-KRT14 antibody
Immunohistochemistry (IHC) Paraffin
Application Notes for anti-KRT14 antibody
Dilution Range: 1:100 - 1:200
Immunohistochemistry (IHC) of anti-KRT14 antibody
IHC (paraffin) of human colon using Cytokeratin 14 Antibody
NCBI/Uniprot data below describe general gene information for KRT14. It may not necessarily be applicable to this product.
NCBI Accession #
EHH24961.1
[Other Products]
UniProt Primary Accession #
P02533
[Other Products]
UniProt Secondary Accession #
Q14715; Q53XY3; Q9BUE3; Q9UBN2; Q9UBN3; Q9UCY4[Other Products]
Molecular Weight
51,561 Da[Similar Products]
NCBI Official Full Name
Cytokeratin-14
UniProt Protein Name
Keratin, type I cytoskeletal 14
UniProt Synonym Protein Names
Cytokeratin-14; CK-14; Keratin-14; K14
UniProt Gene Name
KRT14 [Similar Products]
UniProt Synonym Gene Names
CK-14; K14 [Similar Products]
UniProt Comments for KRT14
The nonhelical tail domain is involved in promoting KRT5-KRT14 filaments to self-organize into large bundles and enhances the mechanical properties involved in resilience of keratin intermediate filaments in vitro.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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