Cytokeratin 14, Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures.

E Coli

Highly Purified
95% by RP-HPLC or SDS-PAGE. Chromatographically purified.

Supplied as a lyophilized powder in 30mM Tris-HCl, pH 8.0, 9.5M urea, 2mM EDTA, 10mM methylammonium chloride. Reconstitute with sterile ddH2O to a concentration >100ug/ml, which can then be further diluted to other aqueous solutions. 0.1% BSA or HSA is re

Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Small volumes of KRT14 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Keratins are a class of fibrous proteins or scleroproteins important both as structural proteins and as keys to the study of protein conformation. The family represents the principal constituent of epidermis, hair, nails, horny tissues, and the organic matrix of tooth enamel. Two major conformational groups have been characterized, alpha-keratin, whose peptide backbone forms an alpha-helix, and beta-keratin, whose backbone forms a zigzag or pleated sheet structure. Cytokeratin 14, a type I intermediate filament, is one of the two cytokeratins that distinguish stratifying epithelial cells types from simple epithelial cell types. Thus, cytokeratin 14 is expressed by stratifying/keratinocyte cell types but not by simple epithelial cells.

Recombinant Human Cytokeratin 14 is a single,non-glycosylated polypeptide chain having a molecular mass of 51,530 Dalton.

Molecular Biology; MB-Keratin, Cytokeratin

~51kD[Similar Products]

Keratin, type I cytoskeletal 14

CK-14; K14  [Similar Products]

K1C14_HUMAN

K14: a type I cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. There are two types of cytoskeletal and microfibrillar keratin: type I (acidic; 40-55 kDa) [K9 to K20] and type II (neutral to basic; 56-70 kDa) [K1 to K8]. Both a basic and an acidic keratin are required for filament assembly. Generally associates with K5.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 17q21.2

Cellular Component: cytoplasm; keratin filament; intermediate filament; nucleus; cytosol

Molecular Function: protein binding; structural constituent of cytoskeleton

Biological Process: epidermis development; hemidesmosome assembly; epithelial cell differentiation; response to zinc ion; response to ionizing radiation; intermediate filament bundle assembly; aging; hair cycle

Disease: Epidermolysis Bullosa Simplex, Localized; Epidermolysis Bullosa Simplex, Generalized; Dermatopathia Pigmentosa Reticularis; Epidermolysis Bullosa Simplex, Autosomal Recessive 1; Naegeli Syndrome; Epidermolysis Bullosa Simplex, Dowling-meara Type

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