TFIIH basal transcription factor complex helicase XPB subunit (ERCC3), ELISA Ki

For Research Use Only. Not for use in diagnostic procedures.

Store all reagents at 2-8 degree C.

We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team

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Small volumes of ERCC3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS7229133 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the TFIIH basal transcription factor complex helicase XPB subunit (ERCC3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ERCC3. The ELISA analytical biochemical technique of the MBS7229133 kit is based on ERCC3 antibody-ERCC3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ERCC3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ERCC3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

89,278 Da

excision repair cross-complementation group 3

TFIIH basal transcription factor complex helicase XPB subunit; BTF2 p89; TFIIH p89; TFIIH 89 kDa subunit; DNA excision repair protein ERCC-3; DNA repair protein complementing XP-B cells; basic transcription factor 2 89 kDa subunit; xeroderma pigmentosum, complementation group B; xeroderma pigmentosum group B-complementing protein; TFIIH basal transcription factor complex 89 kDa subunit; excision repair cross-complementing rodent repair deficiency, complementation group 3

TFIIH basal transcription factor complex helicase XPB subunit

XPB; XPBC; BTF2 p89; TFIIH 89 kDa subunit; TFIIH p89  [Similar Products]

ERCC3_HUMAN

ERCC3 is an ATP-dependent DNA helicase that functions in nucleotide excision repair and complements xeroderma pigmentosum group B mutations. It also is the 89 kDa subunit of basal transcription factor 2 (TFIIH) and thus functions in class II transcription. [provided by RefSeq, Jul 2008]

XPB: ATP-dependent 3'-5' DNA helicase, component of the core- TFIIH basal transcription factor, involved in nucleotide excision repair (NER) of DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. Acts by opening DNA either around the RNA transcription start site or the DNA damage. One of the 6 subunits forming the core-TFIIH basal transcription factor which associates with the CAK complex composed of CDK7, CCNH/cyclin H and MNAT1 to form the TFIIH basal transcription factor. Interacts with PUF60. Interacts with ATF7IP. Interacts with Epstein-Barr virus EBNA2. Belongs to the helicase family. RAD25/XPB subfamily.

Protein type: Transcription factor; EC 3.6.4.12; DNA repair, damage; Helicase

Chromosomal Location of Human Ortholog: 2q21

Cellular Component: nucleoplasm; holo TFIIH complex; nucleus

Molecular Function: protein C-terminus binding; DNA-dependent ATPase activity; GTP binding; ATPase activity; 3'-5' DNA helicase activity; dATP binding; protein N-terminus binding; peptide binding; transcription factor binding; protein kinase activity; ATP-dependent DNA helicase activity; RNA polymerase subunit kinase activity; protein binding; DNA binding; damaged DNA binding; ATP binding

Biological Process: transcription from RNA polymerase II promoter; DNA topological change; viral reproduction; apoptosis; positive regulation of viral transcription; positive regulation of apoptosis; regulation of gene expression, epigenetic; protein amino acid phosphorylation; protein localization; mRNA capping; UV protection; negative regulation of gene expression, epigenetic; transcription-coupled nucleotide-excision repair; nucleotide-excision repair, DNA damage removal; response to UV; transcription initiation from RNA polymerase II promoter; RNA elongation from RNA polymerase I promoter; hair cell differentiation; transcription from RNA polymerase I promoter; nucleotide-excision repair, DNA incision; termination of RNA polymerase I transcription; DNA repair; nucleotide-excision repair, DNA duplex unwinding; nucleotide-excision repair; RNA elongation from RNA polymerase II promoter; response to hypoxia; positive regulation of transcription from RNA polymerase II promoter; gene expression; response to oxidative stress; transcription initiation from RNA polymerase I promoter

Disease: Trichothiodystrophy 2, Photosensitive; Xeroderma Pigmentosum, Complementation Group B

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