KCC3 / SLC12A6, Polyclonal Antibody

Goat Anti-KCC3 / SLC12A6 Antibody; SLC12A6 antibody; solute carrier family 12 (potassium/chloride transporter), member 6 antibody; ACCPN antibody; KCC3 antibody; KCC3A antibody; KCC3B antibody; K-Cl cotransporter 3 antibody; electroneutral potassium-chloride cotransporter 3 antibody; potassium chloride cotransporter 3 antibody; potassium chloride cotransporter KCC3a-S3 antibody; potassium-chloride transporter-3a antibody; potassium-chloride transporter-3b antibody; solute carrier family 12 (potassium/chloride transporters), member 6 antibody; DKFZP434D2135 antibody

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Goat

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Aliquot and store at -20 degree C. Minimize freezing and thawing.

Manufactured in an ISO 9001:2008 Certified Laboratory.

Small volumes of anti-SLC12A6 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)

Western Blot: Approx 120kDa band observed in Human Heart lysates (calculated MW ranging 121-128kDakDa according to NP_598408.1; NP_005126.1; NP_001035959.1; NP_001035960.1; NP_001035961.1; NP_001035962.1). See non-biotinylated parental product's datasheet for further Q
Peptide ELISA: Antibody detection limit dilution 1:16000.

Biotinylated (0.1ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer), exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.

120,467 Da

solute carrier family 12 member 6

solute carrier family 12 member 6

Solute carrier family 12 member 6

KCC3  [Similar Products]

S12A6_HUMAN

This gene is a member of the K-Cl cotransporter (KCC) family. K-Cl cotransporters are integral membrane proteins that lower intracellular chloride concentrations below the electrochemical equilibrium potential. The proteins encoded by this gene are activated by cell swelling induced by hypotonic conditions. Alternate splicing results in multiple transcript variants encoding different isoforms. Mutations in this gene are associated with agenesis of the corpus callosum with peripheral neuropathy. [provided by RefSeq, Jul 2008]

KCC3: Mediates electroneutral potassium-chloride cotransport. May be activated by cell swelling. May contribute to cell volume homeostasis in single cells. Homomultimer and heteromultimer with other K-Cl cotransporters. Up-regulated by VEGF. Down-regulated by TNF. Highly expressed in heart, brain and kidney. Detected at lower levels in skeletal muscle, placenta, lung and pancreas. Detected in umbilical vein endothelial cells. Isoform 2 is more abundant in kidney. Isoform 5 is testis specific. Expressed in the proximal tubule of the kidney. Activated by N-ethylmaleimide (NEM). Inhibited by DIOA, bumetanide and furosemide. Inhibited by WNK3. Belongs to the SLC12A transporter family. 6 isoforms of the human protein are produced by alternative promoter.

Protein type: Transporter; Membrane protein, multi-pass; Transporter, SLC family; Membrane protein, integral

Chromosomal Location of Human Ortholog: 15q13

Cellular Component: basolateral plasma membrane; plasma membrane

Molecular Function: potassium ion symporter activity; potassium:chloride symporter activity

Biological Process: ion transport; synaptic transmission

Disease: Agenesis Of The Corpus Callosum With Peripheral Neuropathy

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