KCC3/SLC12A6, Polyclonal Antibody

SLC12A6; KCC3; solute carrier family 12 (potassium/chloride transporters), member 6; ACCPN; DKFZP434D2135; KCC3A; KCC3B; agenesis of corpus callosum and peripheral neuropathy (Andermann syndrome); potassium chloride cotransporter 3; potassium chloride c; SLC12A6 antibody; KCC3 antibody; solute carrier family 12 (potassium/chloride transporters); member 6 antibody; ACCPN antibody; DKFZP434D2135 antibody; KCC3A antibody; KCC3B antibody; agenesis of corpus callosum and peripheral neuropathy (Andermann syndrome) antibody; potassium chloride cotransporter 3 antibody; potassium chloride c antibody; KCC3 / SLC12A6

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Goat

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100ug specific antibody in 200ul (lot specific)

Aliquot and store at -20 degree C. Minimize freezing and thawing.

Manufactured in an ISO 9001:2008 Certified Laboratory.

Small volumes of anti-SLC12A6 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)

Peptide ELISA: Antibody detection limit dilution 1: 16000.
Immunohistochemistry: In paraffin embedded Human Heart shows staining of myocardial fibrils in transverse section. Recommended concentration, 2-4ug/ml.
Western Blot: Approx 120kDa band observed in Human Heart and Kidney lysates (calculated MW ranging 121-128kDa according to NP_598408.1; NP_005126.1; NP_001035959.1; NP_001035960.1; NP_001035961.1; NP_001035962.1). Recommended concentration: 0.1-0.3ug/ml.

(0.1ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

(2.5ug/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

120,467 Da

solute carrier family 12 member 6

solute carrier family 12 member 6

Solute carrier family 12 member 6

KCC3  [Similar Products]

S12A6_HUMAN

This gene is a member of the K-Cl cotransporter (KCC) family. K-Cl cotransporters are integral membrane proteins that lower intracellular chloride concentrations below the electrochemical equilibrium potential. The proteins encoded by this gene are activated by cell swelling induced by hypotonic conditions. Alternate splicing results in multiple transcript variants encoding different isoforms. Mutations in this gene are associated with agenesis of the corpus callosum with peripheral neuropathy. [provided by RefSeq, Jul 2008]

KCC3: Mediates electroneutral potassium-chloride cotransport. May be activated by cell swelling. May contribute to cell volume homeostasis in single cells. Homomultimer and heteromultimer with other K-Cl cotransporters. Up-regulated by VEGF. Down-regulated by TNF. Highly expressed in heart, brain and kidney. Detected at lower levels in skeletal muscle, placenta, lung and pancreas. Detected in umbilical vein endothelial cells. Isoform 2 is more abundant in kidney. Isoform 5 is testis specific. Expressed in the proximal tubule of the kidney. Activated by N-ethylmaleimide (NEM). Inhibited by DIOA, bumetanide and furosemide. Inhibited by WNK3. Belongs to the SLC12A transporter family. 6 isoforms of the human protein are produced by alternative promoter.

Protein type: Transporter, SLC family; Transporter; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 15q13

Cellular Component: basolateral plasma membrane; integral to membrane; integral to plasma membrane; plasma membrane

Molecular Function: potassium ion symporter activity; potassium ion transmembrane transporter activity; potassium:chloride symporter activity; protein kinase binding

Biological Process: angiogenesis; ion transport; potassium ion import; synaptic transmission

Disease: Agenesis Of The Corpus Callosum With Peripheral Neuropathy

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.