AKR1C3, Polyclonal Antibody

DD3; DDX; HA1753; HAKRB; HAKRe

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit

The antibody detects endogenous level of total AKR1C3 protein.

Antibodies were purified by affinity purification using immunogen.

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

1.0 mg/ml (lot specific)

Store at -20 degree C

Small volumes of anti-AKR1C3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

AKR1C3(Aldo-keto reductase family 1 member C3) is also named as DDH1, HSD17B5, KIAA0119, PGFS and belongsi to AKR1C family..In humans, at least four AKR1C isoforms exist: AKR1C1, AKR1C2, AKR1C3, AKR1C4 and AKR1C3 shares >86% sequence identity with these three highly related human AKRs(PMID:18574251). It catalyzes the conversion of aldehydes and ketones to alcohols and androgen, estrogen, PG, xenobiotics metabolism. The rat kidney possesses a dimeric form of 75 kDa(PMID:18574251).

Total protein Ab

Western Blot (WB), Immunohistochemistry (IHC)

Western blotting: 1:500 - 1:2000
Immunohistochemistry: 1:50 - 1:100

Western blot analysis of extracts of various cell line, using AKR1C3 antibody.

Immunohistochemical analysis of paraffin-embedded human esophageal cancer using AKR1C3 antibody at dilution of 1:100 (200x lens).

Immunohistochemical analysis of paraffin-embedded rat kidney using AKR1C3 antibody at dilution of 1:100 (200x lens).

Immunohistochemical analysis of paraffin-embedded rat brain using AKR1C3 antibody at dilution of 1:100 (200x lens).

23,364 Da

aldo-keto reductase family 1, member C3

aldo-keto reductase family 1 member C3

Aldo-keto reductase family 1 member C3

DDH1; HSD17B5; KIAA0119; PGFS; 17-beta-HSD 5; 3-alpha-HSD type 2; DD-3; DD3; PGFS  [Similar Products]

AK1C3_HUMAN

This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme catalyzes the reduction of prostaglandin (PG) D2, PGH2 and phenanthrenequinone (PQ), and the oxidation of 9alpha,11beta-PGF2 to PGD2. It may play an important role in the pathogenesis of allergic diseases such as asthma, and may also have a role in controlling cell growth and/or differentiation. This gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]

AKR1C3: Catalyzes the conversion of aldehydes and ketones to alcohols. Catalyzes the reduction of prostaglandin (PG) D2, PGH2 and phenanthrenequinone (PQ) and the oxidation of 9-alpha,11-beta- PGF2 to PGD2. Functions as a bi-directional 3-alpha-, 17-beta- and 20-alpha HSD. Can interconvert active androgens, estrogens and progestins with their cognate inactive metabolites. Preferentially transforms androstenedione (4-dione) to testosterone. Belongs to the aldo/keto reductase family.

Protein type: Lipid Metabolism - arachidonic acid; Xenobiotic Metabolism - metabolism by cytochrome P450; EC 1.1.1.149; Oxidoreductase

Chromosomal Location of Human Ortholog: 10p15-p14

Cellular Component: cytoplasm; intracellular; nucleus; cytosol

Molecular Function: 15-hydroxyprostaglandin-D dehydrogenase (NADP+) activity; delta4-3-oxosteroid 5beta-reductase activity; aldo-keto reductase activity; prostaglandin-F synthase activity; 3-alpha(17-beta)-hydroxysteroid dehydrogenase (NAD+) activity; phenanthrene 9,10-monooxygenase activity; 3(or 17)-alpha-hydroxysteroid dehydrogenase activity; retinol dehydrogenase activity; oxidoreductase activity, acting on NADH or NADPH, quinone or similar compound as acceptor; testosterone 17-beta-dehydrogenase (NADP+) activity; ketosteroid monooxygenase activity; trans-1,2-dihydrobenzene-1,2-diol dehydrogenase activity; ketoreductase activity; indanol dehydrogenase activity; aldehyde reductase activity; geranylgeranyl reductase activity; retinal dehydrogenase activity

Biological Process: steroid metabolic process; phototransduction, visible light; retinal metabolic process; male gonad development; cyclooxygenase pathway; progesterone metabolic process; cellular response to starvation; prostaglandin metabolic process; keratinocyte differentiation; positive regulation of protein kinase B signaling cascade; G-protein coupled receptor protein signaling pathway; protein import into nucleus, translocation; positive regulation of cell proliferation; arachidonic acid metabolic process; farnesol catabolic process; multicellular organismal macromolecule metabolic process; retinoid metabolic process; response to nutrient; regulation of retinoic acid receptor signaling pathway

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