Full Product Name
EFNB1 (Phospho-Tyr317) Antibody
Product Synonym Names
CEK5 ligand; CEK5 receptor ligand; CEK5-L; CEL5-L; EFL-3; EFNB1; ELK ligand; ELK-L; EPH-related receptor tyrosine kinase ligand 2; EPL2; EPLG2; Ephrin-B1 precursor; LERK-2; LERK2; STRA1; STRA1 protein; kinase ephrin-B1
Product Gene Name
anti-EFNB1 antibody
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P98172
Species Reactivity
Human, Mouse, Rat
Specificity
EFNB1 (Phospho-Tyr317) Antibody detects endogenous levels of EFNB1 only when phosphorylated at Tyr317.
Purity/Purification
Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Form/Format
Rabbit IgG in phosphate buffered saline (Phospho-without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1 mg/ml (lot specific)
Target Modification
Phospho
Modification Sites
H:Y317; M:Y316; R:Y316
Immunogen
The antiserum was produced against synthesized phospho-peptide derived from human EFNB1 around the phosphorylation site of Tyr317.
Preparation and Storage
Stable at -20 degree C for at least 1 year.
Other Notes
Small volumes of anti-EFNB1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-EFNB1 antibody
Western Blot (WB), ELISA (EIA)
Application Notes for anti-EFNB1 antibody
WB: 1:500~1:1000
ELISA: 1:10000
ELISA (EIA) of anti-EFNB1 antibody
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using EFNB1 (Phospho-Tyr317) Antibody
Western Blot (WB) of anti-EFNB1 antibody
Western blot analysis of lysates from mouse brain, using EFNB1 (Phospho-Tyr317) Antibody.
NCBI/Uniprot data below describe general gene information for EFNB1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_004420.1
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NCBI GenBank Nucleotide #
NM_004429.4
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UniProt Primary Accession #
P98172
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UniProt Secondary Accession #
D3DVU0[Other Products]
UniProt Related Accession #
P98172[Other Products]
NCBI Official Full Name
ephrin-B1
NCBI Official Synonym Full Names
ephrin B1
NCBI Official Symbol
EFNB1 [Similar Products]
NCBI Official Synonym Symbols
CFND; CFNS; EFB1; EFL3; EPLG2; Elk-L; LERK2
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NCBI Protein Information
ephrin-B1
UniProt Protein Name
Ephrin-B1
UniProt Synonym Protein Names
EFL-3; ELK ligand; ELK-L; EPH-related receptor tyrosine kinase ligand 2; LERK-2
UniProt Gene Name
EFNB1 [Similar Products]
UniProt Synonym Gene Names
EFL3; EPLG2; LERK2; ELK-L; LERK-2 [Similar Products]
NCBI Summary for EFNB1
The protein encoded by this gene is a type I membrane protein and a ligand of Eph-related receptor tyrosine kinases. It may play a role in cell adhesion and function in the development or maintenance of the nervous system. [provided by RefSeq, Jul 2008]
UniProt Comments for EFNB1
Binds to the receptor tyrosine kinases EPHB1 and EPHA1. Binds to, and induce the collapse of, commissural axons/growth cones in vitro. May play a role in constraining the orientation of longitudinally projecting axons ().
Research Articles on EFNB1
1. we demonstrate that mosaicism for EPHRIN-B1 expression induced by random X inactivation in heterozygous females results in robust cell segregation in human neuroepithelial cells, thus supplying experimental evidence that Eph/ephrin-mediated cell segregation is relevant to pathogenesis in human CFNS patients.
Precautions
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Disclaimer
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