Full Product Name
PNK Antibody
Product Synonym Names
EIEE10 antibody; MCSZ antibody; PNK antibody; PNKP antibody; DNA 5'-kinase/3'-phosphatase antibody; Homo sapiens polynucleotide kinase 3'-phosphatase (PNKP) antibody; bifunctional polynucleotide phosphatase/kinase antibody; polynucleotide kinase 3'-phosph
Product Gene Name
anti-PNK antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q96T60
Specificity
The antibody detects endogenous levels of total PNK protein.
Purity/Purification
Antibodies were purified by antigen-affinity chromatography.
Form/Format
Rabbit IgG in 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Concentration
0.73 mg/ml (lot specific)
Immunogen Type
Recombinant Protein
Immunogen Description
Recombinant fragment corresponding to a region within amino acids 1 and 273 of PNK.
Preparation and Storage
Store at -20 degree C
Other Notes
Small volumes of anti-PNK antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-PNK antibody
This locus represents a gene involved in DNA repair. In response to ionizing radiation or oxidative damage, the protein encoded by this locus catalyzes 5' phosphorylation and 3' dephosphorylation of nucleic acids. Mutations at this locus have been associated with microcephaly, seizures, and developmental delay.
Product Categories/Family for anti-PNK antibody
Total protein Ab
Applications Tested/Suitable for anti-PNK antibody
Western Blot (WB)
Application Notes for anti-PNK antibody
Western blotting: 1:500-1:3000
Testing Data of anti-PNK antibody
Sample (30 ug of whole cell lysate) A: Hela B: Hep G2 7.5% SDS PAGE PNK antibody diluted at 1:1000
NCBI/Uniprot data below describe general gene information for PNK. It may not necessarily be applicable to this product.
NCBI Accession #
NP_009185.2
[Other Products]
NCBI GenBank Nucleotide #
NM_007254.3
[Other Products]
UniProt Primary Accession #
Q96T60
[Other Products]
UniProt Secondary Accession #
Q9BUL2; Q9P1V2; Q9UKU8; Q9UNF8; Q9UNI0[Other Products]
UniProt Related Accession #
Q96T60[Other Products]
Molecular Weight
53,004 Da
NCBI Official Full Name
bifunctional polynucleotide phosphatase/kinase
NCBI Official Synonym Full Names
polynucleotide kinase 3'-phosphatase
NCBI Official Symbol
PNKP [Similar Products]
NCBI Official Synonym Symbols
PNK; AOA4; MCSZ; EIEE10
[Similar Products]
NCBI Protein Information
bifunctional polynucleotide phosphatase/kinase
UniProt Protein Name
Bifunctional polynucleotide phosphatase/kinase
UniProt Synonym Protein Names
DNA 5'-kinase/3'-phosphatase; Polynucleotide kinase-3'-phosphataseIncluding the following 2 domains:Polynucleotide 3'-phosphatase (EC:3.1.3.32)Alternative name(s):2'(3')-polynucleotidase
UniProt Gene Name
PNKP [Similar Products]
UniProt Entry Name
PNKP_HUMAN
NCBI Summary for PNK
This locus represents a gene involved in DNA repair. In response to ionizing radiation or oxidative damage, the protein encoded by this locus catalyzes 5' phosphorylation and 3' dephosphorylation of nucleic acids. Mutations at this locus have been associated with microcephaly, seizures, and developmental delay.[provided by RefSeq, Sep 2010]
UniProt Comments for PNK
Pnk1: polynucleotide kinase 3'-phosphatase. May be involved in double-strand break repair by non-homologous end joining. Required for normal response to DNA damage by gamma-radiation or camptothecin in S. pombe.
Protein type: EC 2.7.1.78; Kinase, other; EC 3.1.3.32; Phosphatase (non-protein); DNA-binding
Chromosomal Location of Human Ortholog: 19q13.3-q13.4
Cellular Component: nucleoplasm; membrane; nucleolus; nucleus
Molecular Function: protein binding; ATP-dependent polydeoxyribonucleotide 5'-hydroxyl-kinase activity; polynucleotide 3'-phosphatase activity; purine nucleotide binding; endonuclease activity; double-stranded DNA binding; damaged DNA binding; nucleotide kinase activity; ATP binding
Biological Process: response to radiation; dephosphorylation; DNA damage response, detection of DNA damage; nucleotide-excision repair, DNA damage removal; response to oxidative stress; DNA repair; DNA-dependent DNA replication; nucleotide phosphorylation
Disease: Microcephaly, Seizures, And Developmental Delay; Ataxia-oculomotor Apraxia 4
Research Articles on PNK
1. Here we report that purified wild-type (WT) ATXN3 stimulates, and by contrast the mutant form specifically inhibits, PNKP's 3' phosphatase activity in vitro. ATXN3-deficient cells also show decreased PNKP activity
Precautions
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Disclaimer
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