Full Product Name
PNKP Polyclonal Antibody
Product Synonym Names
PNK; MCSZ; EIEE10
Product Gene Name
anti-PNKP antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Purity/Purification
Affinity Purification
Immunogen
Recombinant protein of human PNKP
Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Preparation and Storage
Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Other Notes
Small volumes of anti-PNKP antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-PNKP antibody
This locus represents a gene involved in DNA repair. In response to ionizing radiation or oxidative damage, the protein encoded by this locus catalyzes 5' phosphorylation and 3' dephosphorylation of nucleic acids. Mutations at this locus have been associated with microcephaly, seizures, and developmental delay.
Applications Tested/Suitable for anti-PNKP antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-PNKP antibody
WB: 1:500-1:2000, IHC: 1:50-1:200
Western Blot (WB) of anti-PNKP antibody
Western blot analysis of extracts of various cell lines, using PNKP antibody.
Immunofluorescence (IF) of anti-PNKP antibody
Immunofluorescence analysis of GFP-RNF168 transgenic U2OS cell using PNKP antibody. Green?GFP-RNF168 fusion protein expression for DNA damage marker.Blue: DAPI for nuclear staining. RNF168(GFP) can be used to mark cells damaged by UV-A laser for they always gather around DNA damage region.
NCBI/Uniprot data below describe general gene information for PNKP. It may not necessarily be applicable to this product.
NCBI Accession #
AAH02519.2
[Other Products]
UniProt Secondary Accession #
Q9BUL2; Q9P1V2; Q9UKU8; Q9UNF8; Q9UNI0[Other Products]
UniProt Related Accession #
Q96T60[Other Products]
Molecular Weight
Calculated MW: 57kDa
NCBI Official Full Name
PNKP protein
NCBI Official Synonym Full Names
polynucleotide kinase 3'-phosphatase
NCBI Official Symbol
PNKP [Similar Products]
NCBI Official Synonym Symbols
PNK; AOA4; MCSZ; EIEE10
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NCBI Protein Information
bifunctional polynucleotide phosphatase/kinase
UniProt Protein Name
Bifunctional polynucleotide phosphatase/kinase
UniProt Synonym Protein Names
DNA 5'-kinase/3'-phosphatase; Polynucleotide kinase-3'-phosphataseIncluding the following 2 domains:Polynucleotide 3'-phosphatase (EC:3.1.3.32)Alternative name(s):2'(3')-polynucleotidase
UniProt Gene Name
PNKP [Similar Products]
UniProt Entry Name
PNKP_HUMAN
NCBI Summary for PNKP
This locus represents a gene involved in DNA repair. In response to ionizing radiation or oxidative damage, the protein encoded by this locus catalyzes 5' phosphorylation and 3' dephosphorylation of nucleic acids. Mutations at this locus have been associated with microcephaly, seizures, and developmental delay.[provided by RefSeq, Sep 2010]
UniProt Comments for PNKP
Pnk1: polynucleotide kinase 3'-phosphatase. May be involved in double-strand break repair by non-homologous end joining. Required for normal response to DNA damage by gamma-radiation or camptothecin in S. pombe.
Protein type: EC 3.1.3.32; Kinase, other; EC 2.7.1.78; Phosphatase (non-protein); DNA-binding
Chromosomal Location of Human Ortholog: 19q13.3-q13.4
Cellular Component: membrane; nucleolus; nucleoplasm; nucleus
Molecular Function: ATP binding; ATP-dependent polydeoxyribonucleotide 5'-hydroxyl-kinase activity; damaged DNA binding; double-stranded DNA binding; endonuclease activity; nucleotide kinase activity; polynucleotide 3'-phosphatase activity; protein binding; purine nucleotide binding
Biological Process: base-excision repair; dephosphorylation; DNA damage response, detection of DNA damage; DNA repair; DNA-dependent DNA replication; nucleotide phosphorylation; nucleotide-excision repair, DNA damage removal; positive regulation of telomerase activity; positive regulation of telomere maintenance via telomerase; response to oxidative stress; response to radiation
Disease: Ataxia-oculomotor Apraxia 4; Microcephaly, Seizures, And Developmental Delay
Research Articles on PNKP
1. Here we report that purified wild-type (WT) ATXN3 stimulates, and by contrast the mutant form specifically inhibits, PNKP's 3' phosphatase activity in vitro. ATXN3-deficient cells also show decreased PNKP activity
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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