Beta-NaCH (phospho-Thr615), Polyclonal Antibody

Amiloride-sensitive sodium channel subunit beta; Beta-NaCH; Epithelial Na(+) channel subunit beta; Beta-ENaC; ENaCB; Nonvoltage-gated sodium channel 1 subunit beta; SCNEB

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

IgG

Rabbit

Recognizes endogenous levels of Beta-NaCH (phospho-Thr615) protein.

Immunogen affinity chromatography

Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol

1 mg/mL (lot specific)

Store at -20 degree C.

Small volumes of anti-NaCH antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Phospho-specific Ab

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

WB: 1:500-1:1000
IHC: 1:50-1:100
IF: 1:100-1:300

Western blot analysis of Beta-NaCH (phospho-Thr615) expression in HeLa whole cell lysates.

Immunohistochemical analysis of Beta-NaCH (phospho-Thr615) staining in human brain formalin fixed paraffin embedded tissue section. The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of Beta-NaCH (phospho-Thr615) staining in COS7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a hidified chamber. Cells were washed with PBST and incubated with Alexa Fluor 647-conjugated secondary antibody (red) in PBS at room temperature in the dark.

73 kDa

sodium channel epithelial 1 beta subunit

amiloride-sensitive sodium channel subunit beta

Amiloride-sensitive sodium channel subunit beta

Beta-ENaC; ENaCB  [Similar Products]

Nonvoltage-gated, amiloride-sensitive, sodium channels control fluid and electrolyte transport across epithelia in many organs. These channels are heteromeric complexes consisting of 3 subunits: alpha, beta, and gamma. This gene encodes the beta subunit, and mutations in this gene have been associated with pseudohypoaldosteronism type 1 (PHA1), and Liddle syndrome. [provided by RefSeq, Apr 2009]

Sodium permeable non-voltage-sensitive ion channel inhibited by the diuretic amiloride. Mediates the electrodiffusion of the luminal sodium (and water, which follows osmotically) through the apical membrane of epithelial cells. Plays an essential role in electrolyte and blood pressure homeostasis, but also in airway surface liquid homeostasis, which is important for proper clearance of mucus. Controls the reabsorption of sodium in kidney, colon, lung and sweat glands. Also plays a role in taste perception.

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