Product Name
PNKP - PNK, Polyclonal Antibody
Full Product Name
Anti-PNKP - PNK Antibody
Product Synonym Names
Anti-PNKP Antibody
Product Gene Name
anti-PNKP - PNK antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Species Reactivity
Human. Predicted: Mouse, Rat
Purity/Purification
Affinity purified
Concentration
100ug/100ul (lot specific)
Storage Buffer
PBS, pH 7.4 with 0.02% Sodium Azide
Immunogen
Rabbit polyclonal PNKP (1) antibody was raised against a recombinated human PNKP protein 253-418aa (BC033822).
Preparation and Storage
This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Other Notes
Small volumes of anti-PNKP - PNK antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-PNKP - PNK antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-PNKP - PNK antibody
ELISA titer: 1:20,000-1:80,000
Western blot analysis: 1:500-1:1,000
Immunohistochemistry: 1:100-1:500
Optimal dilutions/concentrations should be determined by the end user.
Testing Data of anti-PNKP - PNK antibody
NCBI/Uniprot data below describe general gene information for PNKP - PNK. It may not necessarily be applicable to this product.
NCBI Accession #
BC033822
[Other Products]
UniProt Secondary Accession #
Q9BUL2; Q9P1V2; Q9UKU8; Q9UNF8; Q9UNI0[Other Products]
UniProt Related Accession #
Q96T60[Other Products]
Molecular Weight
53,004 Da
NCBI Official Full Name
Homo sapiens polynucleotide kinase 3'-phosphatase, mRNA
NCBI Official Synonym Full Names
polynucleotide kinase 3'-phosphatase
NCBI Official Symbol
PNKP [Similar Products]
NCBI Official Synonym Symbols
PNK; MCSZ; EIEE10
[Similar Products]
NCBI Protein Information
bifunctional polynucleotide phosphatase/kinase; DNA 5'-kinase/3'-phosphatase; Homo sapiens polynucleotide kinase 3'-phosphatase (PNKP)
UniProt Protein Name
Bifunctional polynucleotide phosphatase/kinase
UniProt Synonym Protein Names
DNA 5'-kinase/3'-phosphatase; Polynucleotide kinase-3'-phosphataseIncluding the following 2 domains:Polynucleotide 3'-phosphatase (EC:3.1.3.32)Alternative name(s):2'(3')-polynucleotidase
UniProt Gene Name
PNKP [Similar Products]
UniProt Entry Name
PNKP_HUMAN
NCBI Summary for PNKP - PNK
This locus represents a gene involved in DNA repair. In response to ionizing radiation or oxidative damage, the protein encoded by this locus catalyzes 5' phosphorylation and 3' dephosphorylation of nucleic acids. Mutations at this locus have been associated with microcephaly, seizures, and developmental delay.[provided by RefSeq, Sep 2010]
UniProt Comments for PNKP - PNK
Pnk1: polynucleotide kinase 3'-phosphatase. May be involved in double-strand break repair by non-homologous end joining. Required for normal response to DNA damage by gamma-radiation or camptothecin in S. pombe.
Protein type: Kinase, other; EC 2.7.1.78; Phosphatase (non-protein); EC 3.1.3.32; DNA-binding
Chromosomal Location of Human Ortholog: 19q13.3-q13.4
Cellular Component: nucleoplasm; membrane; nucleolus; nucleus
Molecular Function: protein binding; ATP-dependent polydeoxyribonucleotide 5'-hydroxyl-kinase activity; polynucleotide 3'-phosphatase activity; purine nucleotide binding; endonuclease activity; double-stranded DNA binding; damaged DNA binding; nucleotide kinase activity; ATP binding
Biological Process: response to radiation; dephosphorylation; DNA damage response, detection of DNA damage; nucleotide-excision repair, DNA damage removal; response to oxidative stress; DNA repair; DNA-dependent DNA replication; nucleotide phosphorylation
Disease: Microcephaly, Seizures, And Developmental Delay; Ataxia-oculomotor Apraxia 4
Product References and Citations for anti-PNKP - PNK antibody
(1) Mandal,S.M., Hegde,M.L., Chatterjee,A., et al. Role of human DNA glycosylase Nei-like 2 (NEIL2) and single strand break repair protein polynucleotide kinase 3'-phosphatase in maintenance of mitochondrial genome. J. Biol. Chem. 287 (4), 2819-2829 (2012).
Research Articles on PNKP - PNK
1. we show that modest inhibition of PNKP in a PTEN knockout background enhances cellular radiosensitivity, suggesting that such a "synthetic sickness" approach involving the combination of PNKP inhibition with radiotherapy
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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